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proteomic aptamer affinity assays

Russian translation: измерение аффинности аптамеров методами протеомики

00:04 Nov 28, 2014
English to Russian translations [PRO]
Science - Biology (-tech,-chem,micro-)
English term or phrase: proteomic aptamer affinity assays
Two unanticipated limitations emerged from detailed examination of prior described methods for performing single- and multi-plex aptamer based assays, including multiplexed proteomic aptamer affinity assays

""Proteomic Affinity Assay""
Catch-0
133 μL 7.5% streptavidin-agarose slurry in 1xSB17,Tw (40 mM HEPES, 102 mM NaCl, 1 mM EDTA, 5 mM MgCl2, 5 mM KCl, 0.05% Tween-20) was added to wells of the filter plate (0.45 μm Millipore HV plates (Durapore cat# MAHVN4550)). The appropriate 1.1x aptamer mix (all aptamers contain a Cy3 fluorophore and a photo-cleavable biotin moiety on the 5’ end) was thawed followed by vortexing. The 1.1x aptamer mix was then boiled for 10 min, vortexed for 30 s and allowed to cool to 20°C in a water bath for 20 min. The liquid in the filter plates containing the streptavidin agarose slurry was then removed by centrifugation (1000x g for 1 minute). 100 μL aptamer mix was added to the wells of the filter plate (robotically). The mixture was incubated at 25°C for 20 min on a shaker set at 850 rpm, protected from light.

Спасибо!
dao2812
Russian translation:измерение аффинности аптамеров методами протеомики
Explanation:
*
Selected response from:

Natalie
Poland
Local time: 22:07
Grading comment
Спасибо!
4 KudoZ points were awarded for this answer



Summary of answers provided
4измерение аффинности аптамеров методами протеомики
Natalie
4Протеомное определение аффинности аптамеров
Evgeni Kushch


  

Answers


21 mins   confidence: Answerer confidence 4/5Answerer confidence 4/5
измерение аффинности аптамеров методами протеомики


Explanation:
*

Natalie
Poland
Local time: 22:07
Specializes in field
Native speaker of: Russian
PRO pts in category: 4329
Grading comment
Спасибо!
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12 hrs   confidence: Answerer confidence 4/5Answerer confidence 4/5
Протеомное определение аффинности аптамеров


Explanation:
This should be the most used type of translation in RU literature.

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Note added at 24 days (2014-12-22 12:53:40 GMT) Post-grading
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В диссертации использовано словосочетание "определение аффинности аптамеров" http://niibch.ru/files/201407/Dissertation_Zamay_AS.pdf

В другом источнике (электронная библиотека ДВГМУ) применяется слово "протеомные" ("протеомные диагностические технологии")
http://www.fesmu.ru/elib/search.aspx?heading="�������"&page=2

В еще одном источнике употребляется словосочетание "протеомный анализ"

http://niifhm.ru/tezisy-dokladov-itogovoj-nauchno-praktiches...

Считаю абсолютно приемлемым перевод "протеомное определение (измерение, анализ, исследование) аффинности аптамеров"


    Reference: http://webcache.googleusercontent.com/search?q=cache:pmVrKN9...
    Reference: http://webcache.googleusercontent.com/search?q=cache:l8ZTwxN...
Evgeni Kushch
Ukraine
Local time: 23:07
Specializes in field
Native speaker of: Native in RussianRussian, Native in UkrainianUkrainian
PRO pts in category: 217

Peer comments on this answer (and responses from the answerer)
neutral  Natalie: Выражение "протеомное определение" не встречается вообще: http://tinyurl.com/necw939 ("No results found for "Протеомное определение") //Там тоже нет (есть протеомные технологии, анализ, картирование, профилирование итп, но это разные вещи)
4 hrs
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