KudoZ home » English » Biology (-tech,-chem,micro-)

spiked blank recovery

English translation: spiked blank recovery

Advertisement

Login or register (free and only takes a few minutes) to participate in this question.

You will also have access to many other tools and opportunities designed for those who have language-related jobs
(or are passionate about them). Participation is free and the site has a strict confidentiality policy.
17:21 Aug 8, 2006
English to English translations [PRO]
Science - Biology (-tech,-chem,micro-)
English term or phrase: spiked blank recovery
I need a good (i.e. comprehensive to me) explanation of this meat safety testing method. Moreover, I would like to know how this is different from **spiked recovery" also seen in the following sentence.
Following is the context:

As part of the quality control, measurements of the standards (spiked blank recovery) to validate the chromatographic method and measurements of recovery of each analyte from a spiked sample (spiked recovery) were included.
humbird
English translation:spiked blank recovery
Explanation:
Apparently, the amounts of one or more substances in meat samples are being determined by a method involving extraction and chromatography. As extraction is unlikely to extract 100% of the substance(s), a known amount of a similar substace is added to the sample before extraction (this is the spike). The amount of this substance detected on the chromatogram divided by the original amount added gives the recovery, expressed as a % (= spike recovery).
In order to know the amount of substance represented by the chromatography peaks, standards, i.e. solutions containing a range of known amounts of the substance(s) being measured, are processed in the same way as the meat samples (extraction + chromatography). As these standards contain no meat sample material, they are "blanks". Recovery must also be determined for these blank standards, so they are "spiked" by addition of the same spike substance as the meat samples (=> spike blank recovery).
Note: This does not seem to be the usual meaning of "blank", which is a test on a solution containing none of the substance being determined.
Selected response from:

David Sirett
Local time: 02:16
Grading comment
Thank you for your detailed and comprehensive answer. This makes sense.
4 KudoZ points were awarded for this answer

Advertisement


Summary of answers provided
4 +3spiked blank recoveryDavid Sirett


  

Answers


13 hrs   confidence: Answerer confidence 4/5Answerer confidence 4/5 peer agreement (net): +3
spiked blank recovery


Explanation:
Apparently, the amounts of one or more substances in meat samples are being determined by a method involving extraction and chromatography. As extraction is unlikely to extract 100% of the substance(s), a known amount of a similar substace is added to the sample before extraction (this is the spike). The amount of this substance detected on the chromatogram divided by the original amount added gives the recovery, expressed as a % (= spike recovery).
In order to know the amount of substance represented by the chromatography peaks, standards, i.e. solutions containing a range of known amounts of the substance(s) being measured, are processed in the same way as the meat samples (extraction + chromatography). As these standards contain no meat sample material, they are "blanks". Recovery must also be determined for these blank standards, so they are "spiked" by addition of the same spike substance as the meat samples (=> spike blank recovery).
Note: This does not seem to be the usual meaning of "blank", which is a test on a solution containing none of the substance being determined.

David Sirett
Local time: 02:16
Specializes in field
Native speaker of: Native in EnglishEnglish
PRO pts in category: 24
Grading comment
Thank you for your detailed and comprehensive answer. This makes sense.

Peer comments on this answer (and responses from the answerer)
agree  Uma Hariharan
3 hrs

agree  Laura_Fazio
9 hrs

agree  xxxAlfa Trans
12 hrs
Login to enter a peer comment (or grade)




Return to KudoZ list


KudoZ™ translation help
The KudoZ network provides a framework for translators and others to assist each other with translations or explanations of terms and short phrases.



See also:



Term search
  • All of ProZ.com
  • Term search
  • Jobs
  • Forums
  • Multiple search