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packaging construct & yield

French translation: couche d'encapsulation

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GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
English term or phrase:packaging construct & yield
French translation:couche d'encapsulation
Entered by: Mihaela Sinca
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14:56 Jan 25, 2004
English to French translations [PRO]
Tech/Engineering
English term or phrase: packaging construct & yield
a packaging construct having an HIV or MLV gaga/pol before and after concentration (= yield)

une "construction" d'encapsidation ?
ADSTRAD
Local time: 05:08
couche d'encapsulation
Explanation:
The first plasmid is the packaging construct. The backbone of the virus system is the packaging construct (seen above), also known as pCMVAR9. The pCMVAR9 contains human cytomegalovirus (hCMV), which is responsible for the expression of the virus proteins during translation. The packaging signal (the red dot on the SD triangle), that is responsible for activating the packaging process, has been removed. The cis-acting, or self-acting, adjacent sequences are responsible for packaging the viral DNA. Since the packaging signal and the cis-acting sequences have been separated, the packaging sequences will not be incorporated into the viral genome. This means that the envelope proteins will not be produced when the transfer vector is present, which contains the packaging signal, and therefore the virus will not reproduce after it has infected the host cell. The 3' LTR, or long terminal repeat, has been replaced with a polyadenylation signal. The LTR's define the ends of the viral DNA and are responsible for integrating the viral DNA sequence. Replication occurs from the 3' to 5' end, since the 3' LTR has been removed the viral DNA will not integrate and begin replication (5 & 10).




Selected response from:

Mihaela Sinca
Local time: 06:08
Grading comment
merci!
4 KudoZ points were awarded for this answer

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Summary of answers provided
3couche d'encapsulation
Mihaela Sinca


  

Answers


21 hrs   confidence: Answerer confidence 3/5Answerer confidence 3/5
couche d'encapsulation


Explanation:
The first plasmid is the packaging construct. The backbone of the virus system is the packaging construct (seen above), also known as pCMVAR9. The pCMVAR9 contains human cytomegalovirus (hCMV), which is responsible for the expression of the virus proteins during translation. The packaging signal (the red dot on the SD triangle), that is responsible for activating the packaging process, has been removed. The cis-acting, or self-acting, adjacent sequences are responsible for packaging the viral DNA. Since the packaging signal and the cis-acting sequences have been separated, the packaging sequences will not be incorporated into the viral genome. This means that the envelope proteins will not be produced when the transfer vector is present, which contains the packaging signal, and therefore the virus will not reproduce after it has infected the host cell. The 3' LTR, or long terminal repeat, has been replaced with a polyadenylation signal. The LTR's define the ends of the viral DNA and are responsible for integrating the viral DNA sequence. Replication occurs from the 3' to 5' end, since the 3' LTR has been removed the viral DNA will not integrate and begin replication (5 & 10).







    Reference: http://wwwpp.uwrf.edu/~kk00/hivvector/hivvector.html
    Reference: http://biology.kenyon.edu/slonc/gene-web/Lentiviral/Lentivi2...
Mihaela Sinca
Local time: 06:08
Native speaker of: Native in RomanianRomanian
PRO pts in pair: 196
Grading comment
merci!
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