20:45 Apr 11, 2010 

Auf der Internetseite von ThermoFisher wird folgendermaßen formuliert:
Thermo Scientific* Pierce* TMB Substrate Solutions and Kit

PI-34028

Produce faster color development than other HRP substrates due to faster oxidation

TMB (3,3',5,5' tetramethylbenzidine) is a chromagen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) with major absorbances at 370 and 652nm. The color then changes to yellow (measured at 450nm) upon addition of sulfuric or phosphoric acid to stop the reaction. TMB is very sensitive and more quickly oxidized than other HRP substrates, resulting in faster color development. Unlike other commercially available substrates, these products contain no DMF or DMSO. There are three formulations that differ primarily in their sensitivities. Slow TMB is intermediate in sensitivity, ideal for kinetic readings. The sensitivity of the Turbo TMB compares to that of OPD used at approximately 1mg/ml. Ultra TMB-ELISA produces the highest signal:noise ratio and sensitivity in the picogram range.
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