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cycling parameter

German translation: PCR-Bedingungen / Zyklusbedingungen / Zyklusparameter


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GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
English term or phrase:cycling parameter
German translation:PCR-Bedingungen / Zyklusbedingungen / Zyklusparameter
Entered by: Klaus Urban
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19:36 Sep 7, 2010
English to German translations [PRO]
Science - Biology (-tech,-chem,micro-) / Biotechnologie / DNA-Analyse
English term or phrase: cycling parameter
Es geht um die DNA-Analyse der drei ältesten Kamelien Europas, um herauszufinden, ob sie alle derselben Sorte angehören.

"For the molecular marker analysis, DNA was isolated from 50 mg of leaves with EZNA HP Plant DNA Kit according to the manufacturer protocol (Omega Bio-Tek). A set of fourteen polymorphic microsatellites developed in C. japonica (Ueno et al., 1999; Abe et al., 2006) and C. sinensis (Freeman et al., 2004; Hung et al., 2008; Zhao et al., 2008), was tested (Table 2). DNA fragments were amplified using the tailed primer method (Shuelke, 2000). Polymorphisms of 14 microsatellite loci were assessed in a maximum of 95 plants from the living collection of Diputación Provincial de Pontevedra. The reactions were performed in 25 μL total volume containing 50 ng of genomic DNA. ***Cycling parameters*** were as follows: 5 min at 95°C, 25 cycles for 1 min at 95°C, 45 s of hybridization with corresponding Tm (Table 2), 1 min at 72°C, 20 cycles for 1 min at 95°C, 45 s of hybridization at 55°C Tm, 1 min at 72°C and a final step of 60 min at 72°C."
Klaus Urban
Local time: 13:51
PCR-Bedingungen
Explanation:
So würde ich das nennen, als Alternative zu Johannas Version.
Selected response from:

Dr. Matthias Schauen
Germany
Local time: 13:51
Grading comment
Vielen Dank an alle!
4 KudoZ points were awarded for this answer



Summary of answers provided
4ZyklusparameterKonrad Schultz
4PCR-Bedingungen
Dr. Matthias Schauen
4Zyklusbedingungen
Dr. Johanna Schmitt


Discussion entries: 1





  

Answers


10 mins   confidence: Answerer confidence 4/5Answerer confidence 4/5
Zyklusbedingungen


Explanation:
http://www.roche-applied-science.com/pack-insert/1835297b.pd...

Dr. Johanna Schmitt
Germany
Local time: 13:51
Specializes in field
Native speaker of: Native in GermanGerman
PRO pts in category: 44
Notes to answerer
Asker: Danke!!!

Login to enter a peer comment (or grade)

26 mins   confidence: Answerer confidence 4/5Answerer confidence 4/5
PCR-Bedingungen


Explanation:
So würde ich das nennen, als Alternative zu Johannas Version.

Dr. Matthias Schauen
Germany
Local time: 13:51
Specializes in field
Native speaker of: Native in GermanGerman
PRO pts in category: 89
Grading comment
Vielen Dank an alle!
Login to enter a peer comment (or grade)

1 hr   confidence: Answerer confidence 4/5Answerer confidence 4/5
Zyklusparameter


Explanation:
Tabelle 5 in


    Reference: http://www.diss.fu-berlin.de/diss/servlets/MCRFileNodeServle...
Konrad Schultz
Local time: 13:51
Native speaker of: Native in GermanGerman
PRO pts in category: 4
Notes to answerer
Asker: Danke, Konrad!

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Changes made by editors
Sep 7, 2010 - Changes made by Klaus Urban:
Field (specific)Botany => Biology (-tech,-chem,micro-)


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