Login or register (free and only takes a few minutes) to participate in this question.You will also have access to many other tools and opportunities designed for those who have language-related jobs (or are passionate about them). Participation is free and the site has a strict confidentiality policy.
English to German translations [PRO]
Science - Biology (-tech,-chem,micro-) / Biotechnologie / DNA-Analyse | | English term or phrase: 45 s | Es geht um die DNA-Analyse der drei ältesten Kamelien Europas, um herauszufinden, ob sie alle derselben Sorte angehören.
"For the molecular marker analysis, DNA was isolated from 50 mg of leaves with EZNA HP Plant DNA Kit according to the manufacturer protocol (Omega Bio-Tek). A set of fourteen polymorphic microsatellites developed in C. japonica (Ueno et al., 1999; Abe et al., 2006) and C. sinensis (Freeman et al., 2004; Hung et al., 2008; Zhao et al., 2008), was tested (Table 2). DNA fragments were amplified using the tailed primer method (Shuelke, 2000). Polymorphisms of 14 microsatellite loci were assessed in a maximum of 95 plants from the living collection of Diputación Provincial de Pontevedra. The reactions were performed in 25 μL total volume containing 50 ng of genomic DNA. Cycling parameters were as follows: 5 min at 95°C, 25 cycles for 1 min at 95°C, ***45 s*** of hybridization with corresponding Tm (Table 2), 1 min at 72°C, 20 cycles for 1 min at 95°C, ***45 s*** of hybridization at 55°C Tm, 1 min at 72°C and a final step of 60 min at 72°C."
Sind das 45 Sekunden? |
|   Klaus UrbanKudoZ activityQuestions: 1439 ( 10 open) ( 1 without valid answers) ( 26 closed without grading) Answers: 945
| | Local time: 13:51
|
| | 45 s | Explanation:
Ja, 45 Sekunden:
http://www.roche-applied-science.com/pack-insert/1835297b.pd...
Das kann man ja auch einfach so stehen lassen
--------------------------------------------------
Note added at 18 mins (2010-09-07 20:01:27 GMT)
--------------------------------------------------
Hier ist noch ein link:
"PCR amplification was carried out using a Bio-Rad iCycler (Hercules, CA) under the following thermal program: initial denaturation (95°C, 3 min); 30 cycles of 95°C (45 s), 54°C (45 s), and 72°C (45 s); and final extension (72°C, 3 min)"
http://aem.asm.org/cgi/content/full/73/1/73 |
| Selected response from:
Dr. Johanna Schmitt
Germany
Local time: 13:51
| Grading comment Danke!
4 KudoZ points were awarded for this answer |
| |
| Summary of answers provided | | 4 +6 | 45 s | |
| Discussion entries: 0 |
|---|
Automatic update in 00:
|
7 mins confidence:   peer agreement (net): +6 | |
The translation workplace 
