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dye-terminator chemistry

German translation: Dye-Terminator-Methode

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English term or phrase:	dye-terminator chemistry
German translation:	Dye-Terminator-Methode
Entered by:	ClausLSC

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17:43 Dec 11, 2009

English to German translations [PRO]
Tech/Engineering - Chemistry; Chem Sci/Eng / patent application

English term or phrase: dye-terminator chemistry

PCR products were purified and sequenced using dye-terminator chemistry.

ClausLSC KudoZ activity Questions: 58 (none open) (2 without valid answers) (3 closed without grading) Answers: 2
Local time: 09:15

Dye-Terminator-Methode

Explanation:
Googlet besser als "Farbstoff-Terminator-Methode".
Ans Kettenende wird ein fluoreszenzmarkiertes Didesoxynukleotid angehängt statt eines radioaktiv markierten.

Selected response from:

Dr. Matthias Schauen
Germany
Local time: 09:15

Grading comment

Herzlichen Dank für die Hilfe!
4 KudoZ points were awarded for this answer

Summary of answers provided

3 +4

Dye-Terminator-Methode

Dr. Matthias Schauen

Farbstoffabbruchmethode

Konrad Schultz

fluoreszenzmarkierte Kettenabbruchschemie

Christiane Feldmann-Leben

Summary of reference entries provided

Info

Kim Metzger

Answers

5 mins confidence:

fluoreszenzmarkierte Kettenabbruchschemie

Explanation:
Langenscheidt Biotechnologie sagt: dye terminator: fluoreszenzmarkierte Kettenabbruchssequenz
Vielleicht hilft das zumindest weiter.

Christiane Feldmann-Leben
Germany
Local time: 09:15
Specializes in field
Native speaker of:

German
PRO pts in category: 4

7 mins confidence:

peer agreement (net): +4

Dye-Terminator-Methode

Explanation:
Googlet besser als "Farbstoff-Terminator-Methode".
Ans Kettenende wird ein fluoreszenzmarkiertes Didesoxynukleotid angehängt statt eines radioaktiv markierten.

Dr. Matthias Schauen
Germany
Local time: 09:15
Works in field
Native speaker of:

German
PRO pts in category: 88

Grading comment

Herzlichen Dank für die Hilfe!

1 hr confidence:

Farbstoffabbruchmethode

Explanation:
falls es deutsch gewünscht wird, ist immerhin richtig, aber nur in einem Patent auffindbar. Die Erfinder sind schließlich Deutsche.

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Note added at 3 Stunden (2009-12-11 20:52:33 GMT)
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@sibsab: Können wir fortsetzen ;-)) Wer auf diesem Gebiet arbeitet, kennt fast nur englische Arbeiten und braucht gar keine Übersetzung. Und da es um Patentanwendungen geht, geht es womöglich gerade um dieses eine Patent, als einziges deutsches Werk. Da die Patentübersetzerei EN->DE einschläft, wird man den Erfinder wohl fragen "Kannst du kein Englisch?", oder er bezahlt einen Übersetzer DE->EN. Machen wir uns da nichts vor.

Reference: http://www.patent-de.com/20060209/DE102004035543A1.html

Konrad Schultz
Local time: 09:15
Native speaker of:

German
PRO pts in category: 4

Reference comments

13 mins

Reference: Info

Reference information:
Nach einigen Optimierungsversuchen konnte eine für die Dye-Terminatoren-Chemie optimale Primermenge von 0,4μl je Primer (5μM) festgelegt werden, mit der dann nachfolgend in der zweiten PCR gearbeitet wurde.

http://www.certagen.de/docs/Diplom-RP.pdf

Zugabe von fluoreszenzmarkierten Dideoxy-Nucleotiden („Dye-Terminatoren“)
http://studmed.unibe.ch/dozfiles/SGal448Mol.gen._Methoden.pd...

Sequencing with Dye Terminators
Fluorescent DNA sequencing can also be performed using a chemistry in which the dyes are attached to the ddNTPs, thereby requiring only one reaction tube per sample, instead of four. Since only one reaction tube is required for the dye terminator reaction, this chemistry is simpler to use than dye primer chemistry. DNA template, unlabeled primer, buffer, the four dNTPs, the four fluorescently labeled ddNTPs, and AmpliTaq® DNA Polymerase are added to the reaction tube. Fluorescent fragments are generated by incorporation of dye-labeled ddNTPs. Each different ddNTP (ddATP, ddCTP, ddGTP, or ddTTP) will carry a different color of dye. All terminated fragments (those ending with a ddNTP), therefore, contain a dye at their 3’ end.
http://www3.appliedbiosystems.com/AB_Home/applicationstechno...

Kim Metzger
Mexico
Native speaker of:

English
PRO pts in category: 15

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dye-terminator chemistry

German translation: Dye-Terminator-Methode

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dye-terminator chemistry

German translation: Dye-Terminator-Methode

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