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09:37 Sep 13, 2007
English to German translations [PRO] Medical - Genetics / Molekularbiologie
English term or phrase:secondary alone
The antibodies employed have been extensively characterized in the existing literature and widely used (Lee et al. 1987 ; Roberts-Lewis et al. 1994 ; Saatman et al. 1996 ; Posmantur et al. 1997 ; Povlishock et al. 1997 ; Buki et al. 1999a ). Nevertheless, additional control studies were performed to achieve further rigor. To this end, all immunocytochemical reactions included sections incubated without the addition of the primary or secondary antibody or with the **secondary alone** with or without TSA in concert with multiple dilution strategies to obtain optimal staining.