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English to German translations [PRO]
Medical - Medical (general) / Anatomy; In Vitro Diagnostics | | English term or phrase: aqueous mounting media | 4. Wash 4 times in buffer.
5. To reduce nonspecific background staining due to endogenous peroxidase, incubate slide in Hydrogen Peroxide Block for 10-15 minutes.
6. Wash 4 times in buffer.
7. Optional: Apply Ultra V Block and incubate for 5 minutes at room temperature to block nonspecific background staining. NOTE: Do not exceed 10 minutes or there may be a reduction in desired stain. (May be omitted if primary antibodies are diluted in buffers containing 5-10% normal goat serum.)
8. Wash (Optional).
9. Apply primary antibody and incubate according to manufacturer's recommended protocol.
10. Wash 4 times in buffer.
11. Apply UltraVision ONE HRP Polymer and incubate for 30 minutes at room temperature. (NOTE: HRP Polymer is light sensitive. Please avoid unnecessary light exposure and store in opaque vial.)
12. Wash 4 times in buffer.
13. Apply ready-to-use AEC Single Solution to tissue. Incubate for 10 minutes. Modify incubation time to optimize staining in your laboratory.
14. Wash 4 times in DI water.
15. Counterstain and coverslip using an **aqueous mounting media**. |
| | | Selected response from:
  Ingo Dierkschnieder
Local time: 06:06
| Grading comment Danke Ingo.
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