Login or register (free and only takes a few minutes) to participate in this question.You will also have access to many other tools and opportunities designed for those who have language-related jobs (or are passionate about them). Participation is free and the site has a strict confidentiality policy. English to Greek translations [PRO] Science - Food & Dairy / microbiology, chemical reactions | | English term or phrase: fractioning | | For fractioning of aminopeptidase activities, dialysed cheese or micro-organism extracts were submitted to batch chromatography in Q-Sepharose (Amersham), equilibrated in 20 mM sodium phosphate, pH 7.5, in a proportion of one A280 unit of protein per mL packed resin. Samples and resin were mixed in Becker and kept under stirring in an ice bath for 2 h. The mixture was poured into a glass-sintered funnel and washed with the equilibrating buffer to recover nonretained fractions. Three elution steps were carried out, adding NaCl to the equilibrating buffer: fraction I: 150 mM NaCl; fraction II: 300 mM NaCl; and fraction III 500 mM NaCl. The protein content and aminopeptidase activity of fractions were analysed as described. |
| Kyriacos GeorghiouKudoZ activityQuestions: 24 (none open) ( 1 without valid answers) Answers: 419 Cyprus
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| | Selected response from: Annie Xystouris Belgium Local time: 07:46
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