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endonuclease restriction digestion

Latvian translation: DNS molekulas šķelšana, izmantojot restrikcijas endonukleāzes

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13:40 Nov 2, 2007
English to Latvian translations [PRO]
Medical - Medical: Pharmaceuticals
English term or phrase: endonuclease restriction digestion
Synthetic linkers containing one or more restriction sites provide an alternative method of joining the DNA segment to vectors. The DNA segment, generated by endonuclease restriction digestion as described earlier, is treated with bacteriophage T4 DNA polymerase or E. coli DNA polymerase I, enzymes that remove protruding, 3'-single-stranded termini with their 3'-5'-exonucleolytic activities, and fill in recessed 3'-ends with their polymerising activities.
Vents Villers
Local time: 03:20
Latvian translation:DNS molekulas šķelšana, izmantojot restrikcijas endonukleāzes
Explanation:
Visi linki tie paši, tikai nesanāca izrediģēt savu ierosinājumu

What is Restriction Digestion?
Restriction Digestion is the process of cutting DNA molecules into smaller pieces with special enzymes called Restriction Endonucleases (sometimes just called Restriction Enzymes or RE's). These special enzymes recognize specific sequences in the DNA molecule (for example GATATC) wherever that sequence occurs in the DNA.

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Note added at 1 day36 mins (2007-11-03 14:17:01 GMT)
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ar restrikcijas endonukleāžu palīdzību

http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2...

http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=CELEX:...
un citur
Selected response from:

Inese Poga-Smith
Canada
Local time: 20:20
Grading comment
Paldies:)
4 KudoZ points were awarded for this answer

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Summary of answers provided
4DNS molekulas šķelšana, izmantojot restrikcijas endonukleāzes
Inese Poga-Smith


  

Answers


1 day31 mins   confidence: Answerer confidence 4/5Answerer confidence 4/5
DNS molekulas šķelšana, izmantojot restrikcijas endonukleāzes


Explanation:
Visi linki tie paši, tikai nesanāca izrediģēt savu ierosinājumu

What is Restriction Digestion?
Restriction Digestion is the process of cutting DNA molecules into smaller pieces with special enzymes called Restriction Endonucleases (sometimes just called Restriction Enzymes or RE's). These special enzymes recognize specific sequences in the DNA molecule (for example GATATC) wherever that sequence occurs in the DNA.

--------------------------------------------------
Note added at 1 day36 mins (2007-11-03 14:17:01 GMT)
--------------------------------------------------

ar restrikcijas endonukleāžu palīdzību

http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2...

http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=CELEX:...
un citur


    Reference: http://www.life.uiuc.edu/molbio/geldigest/digest.html
Inese Poga-Smith
Canada
Local time: 20:20
Specializes in field
Native speaker of: Latvian
PRO pts in category: 87
Grading comment
Paldies:)
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