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English to Polish translations [PRO] Science - Biology (-tech,-chem,micro-)
English term or phrase:Coon’s modified Ham’s F12
The cells were pooled, washed extensively in PBS and seeded at high density in 35 mm plates (45 x 103 cells / cm2). Culture medium was Coon’s modified Ham’s F12 supplemented with 10%FCS (Mascia Brunelli, Milano, Italy). Maintenance of the chondrocyte phenotype was estimated by the detection of type II collagen after pepsin digestion of the culture supernatant. Cell viability was evaluated by the Trypan-Blue exclusion test. Cell duplication was determined at regular intervals by trypsinization of the culture and cell number quantification. The experiments of stimulation were performed when the primary cultures reached confluence (passage 0).