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15:00 Nov 7, 2007
English to Polish translations [PRO] Science - Biology (-tech,-chem,micro-)
English term or phrase:reached confluence
"The experiments of stimulation were performed when the primary cultures reached confluence (passage 0). "
Cell viability was evaluated by the Trypan-Blue exclusion test. Cell duplication was determined at regular intervals by trypsinization of the culture and cell number quantification. The experiments of stimulation were performed when the primary cultures reached confluence (passage 0). The cells were then cultured for 2 days in the presence of ascorbic acid (50 mg/ml) and incubated thereafter for 20 h in the absence or presence of (rh)IL-1â (5 ng/ml), with or without the addition of various concentrations of hyaluronic acid (HA), compound of the invention (HA-Re) or rhein.