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spotting solution

Russian translation: раствор для переноса [продуктов ПЦР] на мембрану


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English term or phrase:spotting solution
Russian translation:раствор для переноса [продуктов ПЦР] на мембрану
Entered by: Alex Lilo
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14:13 Apr 8, 2008
English to Russian translations [PRO]
Science - Biology (-tech,-chem,micro-) / растворы
English term or phrase: spotting solution
все понятно, но как правильно назвать этот споттинг раствор? спасибо.


QMT Spotting Solutions

For the spotting and binding of DNA-probes onto QMT Epoxy Slides and QMT Aldehyde Slides we recommend the optimized QMT Spotting Solution I, II or III. For QMT Amino Slides Spotting Solutions I, IV and V are recommended. Make your own decision by selecting the best spotting solution appropriate and optimize your experiments and your spotting setup.

Why five different spotting solutions?

We recommend to compare the results of a complete immobilization and hybridization procedure with all 5 spotting solutions compared with e.g. 3x or 5x SSC. The results always depend on your equipment, your probes and your experimental conditions.

* Spotting Solution I is recommended to create relatively small spots with pin and pipetting spotting systems.
* Spotting Solution II shows good results using the Ring-And-Pin Technology.
* Spotting Solution III is recommended to create larger spots compared to solution I with pin and pipetting spotting systems.
* Spotting Solutions IV and V are designed to yield high intensity spots on Amino slides. They are not intended for usage on Epoxy- and Aldehyde slides.

The probes should be dissolved in water and diluted 1:1 with 2x QMT Spotting Solution to yield a 1x spotting solution.

Therefore, the DNA-molecules have to be adjusted to the double of the final spotting concentration according to the recommended values in the following table. For quantitative validations, e.g. gene expression profiles, the DNA-molecules to be immobilized must always be available in excess.
Alex Lilo
Local time: 21:24
раствор для переноса [продуктов ПЦР] на мембрану
раствор для переноса олигонуклеотидов на мембрану

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Local time: 20:24
Grading comment
Наталья, спасибо
4 KudoZ points were awarded for this answer


Summary of answers provided
4 +1раствор для переноса [продуктов ПЦР] на мембрану

Discussion entries: 1



1 hr   confidence: Answerer confidence 4/5Answerer confidence 4/5 peer agreement (net): +1
раствор для переноса [продуктов ПЦР] на мембрану

раствор для переноса олигонуклеотидов на мембрану

Local time: 20:24
Specializes in field
Native speaker of: Native in RussianRussian
PRO pts in category: 3905
Grading comment
Наталья, спасибо

Peer comments on this answer (and responses from the answerer)
agree  Pavel Nikonorkin
1 hr
  -> Спасибо!
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