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English to Spanish translations [PRO] Science - Chemistry; Chem Sci/Eng / med-pharm
English term or phrase:the .......... is eluted from the.............
The concentrated culture filtrate containing PT and FHA is purified by 2 chromatography steps. In the 1st step (adsorption chromatography), hydroxyapatite (HXP) is added which binds to the FHA whilst the PT remains in solution. The FHA is eluted from the HXP. In the 2nd step (affinity chromatography), PT is treated with ASF 4B Sepharose and eluted.