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Me corrijo: los microsomas se lavaron probablemente con el mismo tampón en el que se suspendieron (y no con el tampón de homogeneización) para eliminar la sacarosa. Pero bueno, ese es un detalle sin importancia para tu traducción.
Aunque no lo dicen de manera explícita, del contexto se desprende que, tras la centrifugación diferencial, lavaron los microsomas (probablemente con el tampón de homogeneización). En todo caso, "washed" no encierra nada en especial, significa simplemente
Pooled human liver microsomes from 10 donors were prepared at the Biomedical Research Institute, Human and Animal Bridge Discussion Group (HAB, Chiba, Japan). Human liver samples were legally procured from National Disease Research Interchange (NDRI, Pennsylvania, U.S.A.) through the international partnership between NDRI and HAB. The study was conducted in accordance with the Declaration of Helsinki. Human livers were homogenized with 0.25 M sucrose containing 3mM Tris and 0.1mM EDTA (pH 7.4), and microsomes were isolated by differential centrifugation using an ordinary method. Washed microsomes were resuspended in 100mM Tris–HCl buffer (pH 7.4) containing 1mM EDTA and 20% glycerol at protein concentrations of 20 mg/ml in the livers.