English to Spanish translations [PRO]
Chemistry; Chem Sci/Eng | | English term or phrase: nicking | | Muchas gracias!! |
| Gabriel RKudoZ activityQuestions: 262 ( 2 open) ( 1 closed without grading) Answers: 0
| Local time: 00:54
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| | Spanish translation:corte/digestión | Explanation:
Es el corte o la digestión que se produce por parte de la enzima cuando actúa sobre la molécula. |
| Selected response from:
 Teresa Alvarez
Local time: 23:24
| Grading comment gracias!!
4 KudoZ points were awarded for this answer |
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| Discussion entries: 0 |
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Automatic update in 00:
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4 hrs confidence:  peer agreement (net): +2 corte/digestión
Explanation:
Es el corte o la digestión que se produce por parte de la enzima cuando actúa sobre la molécula.
| Teresa Alvarez
Local time: 23:24
Specializes in field
Native speaker of:  Spanish
PRO pts in category: 4
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| Changes made by editors |
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| Jan 27 - Changes made by Yana Dovgopol: | | Term Context | In addition, libraries of segments of a marker protein can be used to generate a variegated population of polypeptides for screening and subsequent selection of variant marker proteins or segments thereof. For example, a library of coding sequence fragments can be generated by treating a double stranded PCR fragment of the coding sequence of interest with a nuclease under conditions wherein nicking occurs only about once per molecule, denaturing the double stranded DNA, renaturing the DNA to form double stranded DNA which can include sense/antisense pairs from different nicked products, removing single stranded portions from reformed duplexes by treatment with S1 nuclease, and ligating the resulting fragment library into an expression vector. By this method, an expression library can be derived which encodes amino terminal and internal fragments of various sizes of the protein of interest.
Muchas gracias!! => Muchas gracias!! |
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