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Tris (pH 7.6) 0.3 MKCl, 5 mM DTT solution

Spanish translation: (di) solución de Tris 10 mM (pH 7,6), KCl 0,3 M, DTT 5 mM

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English term or phrase:	Tris (pH 7.6) 0.3 MKCl, 5 mM DTT solution
Spanish translation:	(di) solución de Tris 10 mM (pH 7,6), KCl 0,3 M, DTT 5 mM
Entered by:	Yvonne Becker

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01:28 Jan 13, 2012

English to Spanish translations [PRO]
Science - Chemistry; Chem Sci/Eng

English term or phrase: Tris (pH 7.6) 0.3 MKCl, 5 mM DTT solution

Estoy traduciendo un artículo sobre antagonistas del receptor de la progesterona.

"Relative binding affinities

... Tenfold serial dilutions (10 (to the)-6 – 10 (to the)-10) of the compound to be tested were prepared in a 10 mM Tris (pH 7.6) 0.3 MKCl, 5 mM DTT solution. A 100 ml aliquot of each dilution was transferred to a polystyrene tube containing 5 nM [3H] progesterone or [3H] dexamethasone. To each tube either PR containing extracts from baculovirus (20 mg total protein) or GR containing extracts from MDA-231 cells (250 mg total protein) were added and incubated overnight at 4 C. Hydroxylapatite slurry (100 microl) in 10 mM Tris (pH 7.6) and 2 mM DTT were added and the tubes were incubated for an additional 30 min at 4 C, after which they were centrifuged to recover the pellets."

Según Navarro, TRIS = trometamol, pero encuentro muchas referencias en las que se usa "tris" en español
KCl = KCl (cloruro de potasio)
DTT = DTT (probablemente: diclorodifeniltricloroetano)

¿Cuál es la manera correcta de expresarlo en español?

Un millón de gracias

Yvonne Becker KudoZ activity Questions: 3285 (5 open) (1 without valid answers) (64 closed without grading) Answers: 2086
Local time: 23:24

(di) solución de Tris 10 mM (pH 7,6), KCl 0,3 M, DTT 5 mM

Explanation:
Tris es la abreviatura de tris(hidroximetil)aminometano. Normalmente se emplea así también en espanol.

DTT también es una abreviatura (ditiotreitol)

En espanol la molaridad va después del compuesto.