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Science - Chemistry; Chem Sci/Eng | | English term or phrase: custom derived lgG-Fc sandwich ELISA assay | | muchas gracias!!! |
| Gabriel RKudoZ activityQuestions: 262 ( 2 open) ( 1 closed without grading) Answers: 0
| Local time: 00:55
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| | Selected response from:
 Alistair Ian Spearing Ortiz
Local time: 05:55
| Grading comment gracias!
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7 days confidence:   | custom derived lgg-fc sandwich elisa assay ELISA sándwich lgG-Fc derivado especial
Explanation:
No me parece necesario traducir el "assay", ya que está incluído en el nombre "ELISA" y sería redundante.
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| Jan 27 - Changes made by Yana Dovgopol: | | Term Context | [62] Trap DNA molecules were synthesized by starting with the desired amino acid sequence and optimizing the DNA sequence for mammalian system expression. Trap DNA sequences were cloned into a suitable mammalian expression vector (pCpGfree-vitroHmcs) that can be selected using hygromycin and contains MAR/SAR sequences (insulator and boundary regions) and promoters and enhancers for trap expression. Vectors containing trap sequences were transfected into CHO cells by standard transfection methods and the cells were selected for vector integration with hygromycin. Traps were purified from stably transfected cell lines by collecting cell culture medium and purifying by standard methods (protein A column binding). Traps were eluted from protein A by standard methods and quantitated using a custom derived lgG-Fc sandwich ELISA assay.
muchas gracias!!! => muchas gracias!!! |
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