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gene 6 exonuclease

Spanish translation: exonucleasa del gen 6

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GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
English term or phrase:gene 6 exonuclease
Spanish translation:exonucleasa del gen 6
Entered by: AAG-alcaide
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10:56 Feb 19, 2009
English to Spanish translations [PRO]
Medical - Medical: Pharmaceuticals / Biotecnología.
English term or phrase: gene 6 exonuclease
Text:
... and commercially available exonucleases include lambda exonuclease, bacteriophage T7 "gene 6 exonuclease", exonuclease III and Bal 31 nuclease, which ...
Translation:
... y las exonucleasas disponibles comercialmente incluyen la exonucleasa lambda, la "gene 6 exonuclease" del bacteriofago T7, la exonucleasa III y la nucleasa Bal 31, que ...
AAG-alcaide
Spain
Local time: 05:13
exonucleasa del gen 6
Explanation:
Tan sencillo como eso.

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Note added at 6 mins (2009-02-19 11:02:44 GMT)
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Una explicación en inglés aquí:
http://www.jbc.org/cgi/content/abstract/247/1/305
Gene 6 Exonuclease of Bacteriophage T7
I. PURIFICATION AND PROPERTIES OF THE ENZYME
Carol Kerr 1 and Paul D. Sadowski 1

An exonuclease activity was detectable in crude extracts from Escherichia coli 1200 cells (Endo I-, su-) infected with T7 phage bearing amber mutations in gene 3 (T7 endonuclease I) and gene 5 (T7 DNA polymerase). The activity was not detectable if the infecting phage also contained an amber mutation in gene 6. ***That gene 6 is the structural gene for this enzyme *** was shown by the fact that phage bearing a temperature-sensitive mutation in gene 6 induced an exonuclease activity which was more heat labile than the wild type enzyme.

The enzyme has been purified 500-fold, and the purified preparations are essentially free of ribonuclease, endonuclease, DNA polymerase, and exonuclease I activity although they do contain measurable 3'-phosphatase activity, likely the reflection of residual E. coli exonuclease III.

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Note added at 10 mins (2009-02-19 11:06:07 GMT)
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exonucleasa del gen 6 del bacteriófago T7

http://jvi.asm.org/cgi/content/abstract/44/1/235
Bacteriophage T7 defective in the gene 6 exonuclease promotes site-specific cleavages of T7 DNA in vivo and in vitro.
(...)
These cleavages required the products of genes 3 (endonuclease), 4 (DNA primase), and 5 (DNA polymerase). ***However, the product of gene 6 (exonuclease) must be absent. ***
Selected response from:

Andrés Martínez
Spain
Local time: 05:13
Grading comment
Thanks a lot!
4 KudoZ points were awarded for this answer

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Summary of answers provided
5 +4exonucleasa del gen 6
Andrés Martínez
Summary of reference entries provided
Biología molecular en Medicina de Enedina Maldonado - 2002 -
Miguel Armentia

  

Answers


4 mins   confidence: Answerer confidence 5/5 peer agreement (net): +4
exonucleasa del gen 6


Explanation:
Tan sencillo como eso.

--------------------------------------------------
Note added at 6 mins (2009-02-19 11:02:44 GMT)
--------------------------------------------------

Una explicación en inglés aquí:
http://www.jbc.org/cgi/content/abstract/247/1/305
Gene 6 Exonuclease of Bacteriophage T7
I. PURIFICATION AND PROPERTIES OF THE ENZYME
Carol Kerr 1 and Paul D. Sadowski 1

An exonuclease activity was detectable in crude extracts from Escherichia coli 1200 cells (Endo I-, su-) infected with T7 phage bearing amber mutations in gene 3 (T7 endonuclease I) and gene 5 (T7 DNA polymerase). The activity was not detectable if the infecting phage also contained an amber mutation in gene 6. ***That gene 6 is the structural gene for this enzyme *** was shown by the fact that phage bearing a temperature-sensitive mutation in gene 6 induced an exonuclease activity which was more heat labile than the wild type enzyme.

The enzyme has been purified 500-fold, and the purified preparations are essentially free of ribonuclease, endonuclease, DNA polymerase, and exonuclease I activity although they do contain measurable 3'-phosphatase activity, likely the reflection of residual E. coli exonuclease III.

--------------------------------------------------
Note added at 10 mins (2009-02-19 11:06:07 GMT)
--------------------------------------------------

exonucleasa del gen 6 del bacteriófago T7

http://jvi.asm.org/cgi/content/abstract/44/1/235
Bacteriophage T7 defective in the gene 6 exonuclease promotes site-specific cleavages of T7 DNA in vivo and in vitro.
(...)
These cleavages required the products of genes 3 (endonuclease), 4 (DNA primase), and 5 (DNA polymerase). ***However, the product of gene 6 (exonuclease) must be absent. ***

Andrés Martínez
Spain
Local time: 05:13
Specializes in field
Native speaker of: Spanish
PRO pts in category: 405
Grading comment
Thanks a lot!

Peer comments on this answer (and responses from the answerer)
agree  Miguel Armentia
5 mins
  -> Gracias, Miguel.

agree  Mónica Belén Colacilli
12 mins
  -> Gracias, Mónica.

agree  Teresa Mozo
14 mins
  -> Gracias, Teresa.

agree  Andrea Ali
27 mins
  -> Gracias, Andrea.
Login to enter a peer comment (or grade)




Reference comments


10 mins
Reference: Biología molecular en Medicina de Enedina Maldonado - 2002 -

Reference information:
... o por amplificación por PCR estándar de las secuencias repetidas y digestión de una de las cadenas por la exonucleasa del fago T7 gene 6 (Exo-T7-g6). ...
http://books.google.com/books?id=CEggYSJT8h0C&pg=PA55&lpg=PA...

Saludos

Miguel Armentia
Spain
Specializes in field
Native speaker of: Spanish
PRO pts in category: 158
Login to enter a peer comment (or grade)




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