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19:16 Mar 24, 2007
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German to English translations [PRO] Science - Medical: Pharmaceuticals
German term or phrase:Einzelnachweis
Will man bestimmte Einzelnachweise in einer multiplex PCR zusammenfassen, erfordert dies oft noch eine weitere Optimierung.
Simultaneous detection of E1a, cosmid, and Luc or L3 was not possible because of artifacts observed in the quantification values when compared with the single detections run as controls.
In this report, we first demonstrate that the RT-PCR-RFLP was sensitive in detecting both Massachusetts 41 (Mass 41) and JMK strains of IBV singly; the detection limit for both was approximately 0.15 ng viral RNA using gel electrophoresis.