http://www.drugfuture.com/Pharmacopoeia/USP32/pub/data/v3227...
intradermal injection induction phase
This phase requires three pairs of injections administered intradermally, with the test and control injection of each pair on opposite sides intrascapularly. Each injection should contain 0.1 mL, with injection pairs 1 and 2 administered nearer to the head, and injection pair 3 administered slightly farther towards the tail. The pairs are nominally within an area of 8 cm2. The pairs of injections consist of the following:
Injection pair 1: A 1:1 (v/v) mixture of Freund's Complete Adjuvant (FCA), an oil–water emulsion containing mycobacteria, and the appropriate solvent/vehicle (see Biological Reactivity Tests, In Vivo 88). Control animals receive a mixture of FCA and physiological saline (1:1).
Injection pair 2: The Test Article Preparation in the concentration as specified in Determination of Test Article Concentration, using the appropriate solvent/vehicle. Control animals receive only the solvent/vehicle.
Injection pair 3: The Test Article Preparation in the concentration as specified in Determination of Test Article Concentration in a 1:1 (v/v)
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