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tecnica fenolo-cloroformio da campioni umani di buffy coat

English translation: phenol-chloroform extraction of human buffy coat samples

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18:30 Aug 9, 2004
Italian to English translations [Non-PRO]
Medical - Medical: Pharmaceuticals
Italian term or phrase: tecnica fenolo-cloroformio da campioni umani di buffy coat
Tecniche apprese durante un esperimento in laboratorio.
Marcella
English translation:phenol-chloroform extraction of human buffy coat samples
Explanation:
Buffy coat - a white blood cell preparation.
Phenol chloroform extraction- a very caustic extraction medium which denatures almost all proteins etc, leaving nucleotides intact,hence routinely used for the isolation of RNA. I would guess this refers to the isolation of total RNA from human buffy coat fractions.

Refs - 20 years hands -on experience.
Selected response from:

Alexander Chisholm
Local time: 08:55
Grading comment
4 KudoZ points were awarded for this answer

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Summary of answers provided
5 +1phenol-chloroform extraction of human buffy coat samples
Alexander Chisholm


  

Answers


1 hr   confidence: Answerer confidence 5/5 peer agreement (net): +1
phenol-chloroform extraction of human buffy coat samples


Explanation:
Buffy coat - a white blood cell preparation.
Phenol chloroform extraction- a very caustic extraction medium which denatures almost all proteins etc, leaving nucleotides intact,hence routinely used for the isolation of RNA. I would guess this refers to the isolation of total RNA from human buffy coat fractions.

Refs - 20 years hands -on experience.

Alexander Chisholm
Local time: 08:55
Specializes in field
Native speaker of: English
PRO pts in category: 40

Peer comments on this answer (and responses from the answerer)
agree  Elena Petelos: ...faster...extraction/technique, it's the same procedure...:-)
4 mins
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