English translation: Incubation and culture of dilutions
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A fixed amount of the incubated dilution of the type/reference strain, perhaps 1 mL or perhaps only a 50-µL loopful, was seeded onto a sab plate and allowed to spread naturally over the plate or was streaked out using the loop (the plate was then incubated at 30ºC, not 300ºC). The previous, now hidden, suggestion of ‘seeding’ could be used, but here is another option.
'Szczep muzealny' is not always the same as 'type strain' or 'reference strain', but in this case it looks like ATCC 10231 was used as a type or reference strain, and was not just one of many strains taken from one or other culture collection and tested.
Thanks a lot. Yeah, the temp was 30 not 300, a spello. So "szczep muzealny" can also be "type/reference strain" 4 KudoZ points were awarded for this answer
Z każdego rozcieńczenia szczepu muzealnego Candida albicans ATCC 10231 pobrano po 1ml inokulum, które dodano do 9ml wodnego roztworu koloidu miedzi o stężeniu 10 ppm. Z tak przygotowanych roztworów wykonano wysiewy po 15 minutach, 3 i 5 godzinach od wprowadzenia inokulum do roztworu wody z koloidem miedzi o stężeniu 10ppm. Wysiewu dokonywano na podłoże wybiórcze dla szczepu Candida albicans ( Sabouraud ), które inkubowano w temperaturze +300 C przez 72h. Po zakończeniu inkubacji policzono na wszystkich płytkach wyrosłe kolonie.
Odpowiedzenie na to pytanie jest utrudnione z powodu braku szerszego kontekstu. Jak wyglądały te wysiewy – jakie drobnoustroje wysiewano, jakich rodzajów podłoży używano, czym i jak wprowadzono badany materiał na (do) podłoże itd?
Wysiew, na pewno wysiew...Ale w żadnym wypadku nie z rolnictwa tylko z badań mikrobiologicznych koloidu srebra i miedzi ;) Dlatego też seeding albo sowing raczej nie, a może inoculation? Przydał by się ktoś z tej dziedziny ;)
Warto by wyjaśnić z autorem, czy czasem nie chodziło o "posiew"...
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Incubation and culture of dilutions
Explanation:
A fixed amount of the incubated dilution of the type/reference strain, perhaps 1 mL or perhaps only a 50-µL loopful, was seeded onto a sab plate and allowed to spread naturally over the plate or was streaked out using the loop (the plate was then incubated at 30ºC, not 300ºC). The previous, now hidden, suggestion of ‘seeding’ could be used, but here is another option.
'Szczep muzealny' is not always the same as 'type strain' or 'reference strain', but in this case it looks like ATCC 10231 was used as a type or reference strain, and was not just one of many strains taken from one or other culture collection and tested.
Peter Nicholson Poland Local time: 06:14 Specializes in field Native speaker of: English PRO pts in category: 60
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Thanks a lot. Yeah, the temp was 30 not 300, a spello. So "szczep muzealny" can also be "type/reference strain"
Notes to answerer
Asker: Yeah, looks like it. Thank you very much for the help.
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