Spanish: ADN templadoEnglish translation: template DNA KudoZ The KudoZ network provides a framework for translators ... More |
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Spanish to English translations [PRO] Science - Biology (-tech,-chem,micro-) / Biotechnology studies | | Spanish term or phrase: ADN templado | ¿¿En este caso se refiere a la temperatura del DNA, o a algún procedimiento especial??
La mezcla de reacción usada para la PCR contenía: 100 ng de ******ADN templado****** de P. capsici, 0.2 mM de cada iniciador, 2.5 mM de cada dNTP, 15 mM MgCl2, 100 mM Tris-HCl (pH 8), 500 mM KCl, 3 unidades de Taq polimerasa (Boehringer, Mannheim, Indianapolis, 1N), en un volumen final de 50 l (Medina, 2002). |
| | | template DNA | Explanation: Could it be refering to a template strand of DNA to be used in a PCR (polimerase chain reaction)?
I used to work in a lab doing PCRs only and we used DNA templates to replicate it.
-------------------------------------------------- Note added at 59 mins (2005-12-07 23:54:55 GMT) --------------------------------------------------
Protocol for PCR using Taq DNA PolymeraseA control reaction, omiting template DNA, should always be performed, ... The number of PCR cycles depends on the amount of ***template DNA*** in the reaction mix ...
www.fermentas.com/techinfo/pcr/dnaamplprotocol.htm
Revista chilena de infectolog - <I>Biolog molecular en ... - [ Translate this page ]
Cada hebra de ***ADN (templado)*** sirve como molde para la amplificación y sólo ...
El ADN doble hebra formado (templado: partidor) definirá el sitio de acción ...
http://www.scielo.cl/scielo.php?pid=S0716-10182002000100003&...
Preparation of ***Template for DNA Sequencing***The quality of the DNA template is one of the most critical factors in ... If you have enough template DNA, always determine its concentration by UV ...
seqcore.brcf.med.umich.edu/doc/dnaseq/dnaprep.html
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| Selected response from:
PaulinaRich United States
| Note from asker to answerer¡Muchas gracias, Paulina y a todos por su ayuda! 4 KudoZ points were awarded for this answer |
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2 mins confidence:   |
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