enzyme induced epitope retrieval

14:35 Sep 16, 2011
This question was closed without grading. Reason: Answer found elsewhere

English to Dutch translations [PRO]
Medical - Medical (general)
English term or phrase: enzyme induced epitope retrieval
No difference in amplification status was observed when the enzyme induced epitope retrieval (EIER) enzyme concentration was performed with enzyme concentrate/enzyme diluent ratios of 1:200 and 1:500
Rik Schraag
Local time: 02:52

Summary of reference entries provided
Barend van Zadelhoff


Reference comments

1 hr

Reference information:
deze link gaat over HIER, een direct verwante procedure, en voorziet in interessante achtergrondinformatie en kan je helpen bij het vinden van een acceptabele vertaling als je de hele pagina leest / ik heb weleens epitoopherstel gebruikt maar ik twijfel er nu een beetje aan
het kan ook handig zijn de naam van de produre, Enzyme Induced Epitope Retrieval, onvertaald te laten met een Nederlandse vertaling tussen haakjes erachter

jouw zin is volgens mij een beetje complex geformuleerd

je hebt een procedure: EIER
volgens mij staat er dat de enzymconcentratie waarmee de procedure wordt uitgevoerd een enzymconcentratie is van 1:200 en 1:500
uiteraard wordt er bij de procedure met deze naam een enzym gebruikt (enzyme induced)

Loosely defined, heat-induced epitope retrieval (HIER) is a pretreatment procedure often used prior to immunohistochemistry (IHC) or in situ hybridaization (ISH) procedures to improve staining by modifying the molecular conformation of ‘target’ proteins through an exposure of slide-mounted specimen material (sectioned tissue and other cellular preparations) to a heated buffer solution. This modification process is necessary because, although aldehyde-based fixatives are excellent for preserving cellular morphology, they also cause protein cross-linking, resulting in the inability of some protein epitopes to bind complementary antibodies. HIER is commonly used in conjunction with enzyme digestion as a means of improving the reactivity of various antigens within IHC/ISH staining reactions


Barend van Zadelhoff
Specializes in field
Native speaker of: Native in DutchDutch
PRO pts in category: 277
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