trityl-on/off

Polish translation: metody "z trytylem" i "bez trytylu"

21:46 Feb 6, 2017
English to Polish translations [PRO]
Science - Chemistry; Chem Sci/Eng / organic
English term or phrase: trityl-on/off
Crude synthetic oligonucleotides are purified by either trityl-on or trityl-off methodologies. The trityl-on method offers several key advantages, the most attractive being that it involves fewer steps and is more compatible with automation and high-throughput applications.
Robert Pranagal
Local time: 19:48
Polish translation:metody "z trytylem" i "bez trytylu"
Explanation:
Wiem że są zwolennicy zachowywania angielskich nazw w polskim piśmiennictwie, ale sam jestem zwolennikiem ich tłumaczenia w możliwie najprostszy sposób. Doczytałem o co chodzi, sam wiem z doświadczenia że łatwiej się pracuje na związkach mniej polarnych, tak jak tu z grupą ochronną DMT ("z trytylem"), niż bez niej, ale wszystko ma swoje plusy dodatnie i ujemne ;-)
Selected response from:

kamilw
Local time: 19:48
Grading comment
dzięki

4 KudoZ points were awarded for this answer



Summary of answers provided
3 +1metody "z trytylem" i "bez trytylu"
kamilw
Summary of reference entries provided
Triphenylmethane
M.A.B.
trityl-on/off
Frank Szmulowicz, Ph. D.

  

Answers


3 hrs   confidence: Answerer confidence 3/5Answerer confidence 3/5 peer agreement (net): +1
metody "z trytylem" i "bez trytylu"


Explanation:
Wiem że są zwolennicy zachowywania angielskich nazw w polskim piśmiennictwie, ale sam jestem zwolennikiem ich tłumaczenia w możliwie najprostszy sposób. Doczytałem o co chodzi, sam wiem z doświadczenia że łatwiej się pracuje na związkach mniej polarnych, tak jak tu z grupą ochronną DMT ("z trytylem"), niż bez niej, ale wszystko ma swoje plusy dodatnie i ujemne ;-)

kamilw
Local time: 19:48
Specializes in field
Native speaker of: Native in PolishPolish
PRO pts in category: 305
Grading comment
dzięki

Peer comments on this answer (and responses from the answerer)
agree  Jakub Kościelniak: Brzmi bardzo rozsądnie. Zwłaszcza pisownia trytylu przez 'y'. ;-)
8 hrs
  -> dzięki ;-)
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Reference comments


19 mins
Reference: Triphenylmethane

Reference information:
wiki

Example sentence(s):
  • Triphenylmethane, or triphenyl methane, is the hydrocarbon with the formula (C6H5)3CH. This colorless solid is soluble in nonpolar organic solvents and not in water. Triphenylmethane is the basic skeleton of many synthetic dyes called triarylmethane dyes,

    https://en.wikipedia.org/wiki/Triphenylmethane
M.A.B.
Poland
Specializes in field
Native speaker of: Native in PolishPolish
PRO pts in category: 102
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3 hrs
Reference: trityl-on/off

Reference information:
oczyszczanie oligomeru dzięki lipofilowym właściwościom grupy dimetoksytrytylowej (metoda „trityl on”)
In trityl-on purification protocols, the final 5´ DMT protecting group is retained on the nucleotide and removed during purification.

kontrolowanie wydajności reakcji fosforylacji dzięki uwalnianiu barwnego kationu dimetoksytrytylowego (metoda „trityl off”)
In trityl-off applications, the 5´ protecting group is cleaved in the oligonucleotide synthesizer prior to purification.

ccccc
kwasolabilna
grupa dimetoksytrytylowa
ccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc
By incorporating dimethoxytrityl (DMT), an acid labile 5´ protecting group, nucleotide assembly is accomplished through sequential automated cycles of deprotection, coupling, capping, and oxidation. The greatest potential for damage to the sequence occurs during deprotection (detritylation) where the oligonucleotide is exposed to dilute acid, increasing the risk of purine hydrolysis. Adding further complexity, the depurination rate of single-stranded DNA (as synthetically produced) is fourfold greater than native double-stranded DNA.

In trityl-on purification protocols, the final 5´ DMT protecting group is retained on the nucleotide and removed during purification.

In trityl-off applications, the 5´ protecting group is cleaved in the oligonucleotide synthesizer prior to purification.
http://www.genengnews.com/gen-articles/improving-synthetic-o...
ccccccccccccccccccccc

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Note added at 3 hrs (2017-02-07 01:06:35 GMT)
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Polski artykuł
Michał Sobkowski
CHEMICZNA SYNTEZA
NIERADIOIZOTOPOWYCH SOND
MOLEKULARNYCH
oczyszczanie oligomeru dzięki lipofilowym właściwościom grupy dimetoksytrytylowej (metoda „trityl on”)
kontrolowanie wydajności reakcji fosforylacji dzięki uwalnianiu barwnego kationu dimetoksytrytylowego (metoda „trityl off”)
http://users.man.poznan.pl/msob/doktorat/Doktorat.pdf

Frank Szmulowicz, Ph. D.
United States
Specializes in field
Native speaker of: Native in EnglishEnglish, Native in PolishPolish
PRO pts in category: 518
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