flow bead immunoassays

Portuguese translation: ensaios imunológicos de citometria de fluxo baseada em contas / microesferas

GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
English term or phrase:flow bead immunoassays
Portuguese translation:ensaios imunológicos de citometria de fluxo baseada em contas / microesferas
Entered by: Edna Almeida

15:02 Dec 18, 2020
English to Portuguese translations [PRO]
Other
English term or phrase: flow bead immunoassays
Related to a reagent.
Edna Almeida
Ireland
Local time: 03:45
ensaios imunológicos de citometria de fluxo baseada em contas / microesferas
Explanation:
immunoassays: ensaios imunológicos
https://www.deepl.com/translator#en/pt/immuno assays

From what I could gather, "flow bead" is related to flow cytometry. This could be a good starting point:

"Cell counting using flow cytometry can be accomplished by adding an internal microsphere counting standard to the flow cytometric sample. The number of reference beads that are collected reflects a known volume. This allows you to calculate cell concentration."

The question now is how to translate "bead". I see two options and I found evidence of both:

1) contas:
"Com base na tecnologia, o mercado global é segmentado em citometria de fluxo baseada em células e baseada em contas. Estima-se que o segmento de citometria de fluxo baseada em contas presencie o maior crescimento durante o período de previsão. Essa tecnologia oferece várias vantagens processuais em relação a outros ensaios baseados em células, como a capacidade de detectar vários analitos (também conhecidos como multiplexação), alta reprodutibilidade, estabilidade e velocidade." -- https://icrowdpt.com/2020/12/08/mercado-de-citometria-de-flu...

2) microesferas (as deducted from the excerpt below):

"CountBright™ absolute counting beads are a calibrated suspension of microspheres that are brightly fluorescent across a wide range of excitation and emission wavelengths (UV to 635 nm excitation and 385-800 nm emission). CountBright™ absolute counting beads are mixed with the cell sample and assayed via flow cytometry. By comparing the ratio of bead events to cell events, absolute numbers of cells in the sample can be calculated. Because CountBright™ beads are mixed in the test sample, absolute cell counts using this single-platform method are more accurate and less complicated than cell concentration determined using multiple-platform testing. CountBright™ absolute counting beads can be used with any sample type, including no-wash/lysed whole blood." -- https://www.thermofisher.com/order/catalog/product/C36950#/C...

If at all possible, I suggest consulting the client for clarification on the meaning.

--------------------------------------------------
Note added at 8 hrs (2020-12-18 23:27:24 GMT)
--------------------------------------------------

This article mentions the "microesferas":
http://www.icb.usp.br/~imunoicb/?page_id=1245
Selected response from:

Oliver Simões
United States
Local time: 20:45
Grading comment
Obrigada.
4 KudoZ points were awarded for this answer



Summary of answers provided
2ensaios imunológicos de citometria de fluxo baseada em contas / microesferas
Oliver Simões


  

Answers


8 hrs   confidence: Answerer confidence 2/5Answerer confidence 2/5
ensaios imunológicos de citometria de fluxo baseada em contas / microesferas


Explanation:
immunoassays: ensaios imunológicos
https://www.deepl.com/translator#en/pt/immuno assays

From what I could gather, "flow bead" is related to flow cytometry. This could be a good starting point:

"Cell counting using flow cytometry can be accomplished by adding an internal microsphere counting standard to the flow cytometric sample. The number of reference beads that are collected reflects a known volume. This allows you to calculate cell concentration."

The question now is how to translate "bead". I see two options and I found evidence of both:

1) contas:
"Com base na tecnologia, o mercado global é segmentado em citometria de fluxo baseada em células e baseada em contas. Estima-se que o segmento de citometria de fluxo baseada em contas presencie o maior crescimento durante o período de previsão. Essa tecnologia oferece várias vantagens processuais em relação a outros ensaios baseados em células, como a capacidade de detectar vários analitos (também conhecidos como multiplexação), alta reprodutibilidade, estabilidade e velocidade." -- https://icrowdpt.com/2020/12/08/mercado-de-citometria-de-flu...

2) microesferas (as deducted from the excerpt below):

"CountBright™ absolute counting beads are a calibrated suspension of microspheres that are brightly fluorescent across a wide range of excitation and emission wavelengths (UV to 635 nm excitation and 385-800 nm emission). CountBright™ absolute counting beads are mixed with the cell sample and assayed via flow cytometry. By comparing the ratio of bead events to cell events, absolute numbers of cells in the sample can be calculated. Because CountBright™ beads are mixed in the test sample, absolute cell counts using this single-platform method are more accurate and less complicated than cell concentration determined using multiple-platform testing. CountBright™ absolute counting beads can be used with any sample type, including no-wash/lysed whole blood." -- https://www.thermofisher.com/order/catalog/product/C36950#/C...

If at all possible, I suggest consulting the client for clarification on the meaning.

--------------------------------------------------
Note added at 8 hrs (2020-12-18 23:27:24 GMT)
--------------------------------------------------

This article mentions the "microesferas":
http://www.icb.usp.br/~imunoicb/?page_id=1245

Oliver Simões
United States
Local time: 20:45
Native speaker of: Native in PortuguesePortuguese, Native in EnglishEnglish
PRO pts in category: 72
Grading comment
Obrigada.
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