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Vector stocks

11:46 Oct 26, 2018
This question was closed without grading. Reason: No acceptable answer

English to Russian translations [PRO]
Science - Biology (-tech,-chem,micro-) / иммунология
English term or phrase: Vector stocks
***Vector stocks*** of VSV- G pseudotyped LVs were prepared by 3-plasmid calcium phosphate-mediated transfection in 293T cells.
The use of a SIN-LV vector design allowed a relatively easy and safe production of viral ***stocks***, able to efficiently transduce HSCs; 2) the use of a weak and eukaryotic promoter such as hPGK, which is less susceptible to silencing by methylation, leads to a more physiological expression of the transgene, achieving therapeutic levels with a viral dosage (1.65 VCN) within the clinical standards; and 3) the codon optimized transgene sequence and the presence of the mutated Wpre sequence increase transgene mRNA stability: no reporter gene was included in the therapeutic vector sequence, avoiding possible immunogenic problems.
Local time: 16:10

Summary of answers provided
2раствор на основе вирусного вектора
Ilya Bykov

Discussion entries: 1



1 hr   confidence: Answerer confidence 2/5Answerer confidence 2/5
vector stocks
раствор на основе вирусного вектора

Вирусный вектор - инструмент, обычно используемый молекулярными биологами, чтобы поставить генетический материал в клетки.
Ilya Bykov
Russian Federation
Local time: 17:10
Works in field
Native speaker of: Native in RussianRussian
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