\"in-tandem\" blocking assay

03:24 Jan 15, 2019
English to Russian translations [PRO]
Science - Science (general)
English term or phrase: \"in-tandem\" blocking assay
inhibiting the binding of phosphatidylserine to TIM3, e.g., as measured by PS-hTIM3 "in-tandem" blocking assay
Local time: 10:25

Summary of answers provided
Igor Andreev



6 hrs   confidence: Answerer confidence 3/5Answerer confidence 3/5

назвал бы это методом конкурентного связывания

см. напр.:
In the "tandem blocking assay", TIM3 (ECD)-Fc was captured on Octet biosensor, and anti-TIM3 antibody and PS-liposomes were allowed to bind to the TIM3 protein. When anti-TIM3 binds to a region that is blocking PS binding, PS-liposome shows no binding.

Конкурентный метод. В данном методе используется реакция между антигенами, связанными с твердой фазой и конкурирующими между собой меченными ферментом антителами (конъюгатом) и антителами опытной пробы. Конъюгата связывается тем меньше, чем больше специфических антител в пробе. После удаления несвязавшихся компонентов в ходе промывки измеряют ферментативную активность иммобилизованного комплекса.

Igor Andreev
Local time: 10:25
Native speaker of: Native in RussianRussian
PRO pts in category: 32
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