SA‐AP or AP‐polymer incubation

Spanish translation: incubación del polímero con estreptavidina-fosfatasa alcalina o FA

GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
English term or phrase:SA‐AP or AP‐polymer incubation
Spanish translation:incubación del polímero con estreptavidina-fosfatasa alcalina o FA
Entered by: María Patricia Arce

03:01 Aug 15, 2020
English to Spanish translations [PRO]
Medical - Medical: Instruments / loosely capped slide jar
English term or phrase: SA‐AP or AP‐polymer incubation
Buenos días,

Espero que puedan ayudarme con una expresión al español: “SA‐AP or AP‐polymer incubation”, relacionada con un kit de tinción. Muchas gracias:

“Staining Procedure:

- After SA‐AP or AP‐polymer incubation, wash tissue sections wit buffer.
- Wipe glass, removing excess buffer. Add drops of Perma/AP working solution to cover sections.”

GVL
Giovanni VL
Local time: 22:08
incubación del polímero con estreptavidina-fosfatasa alcalina o FA
Explanation:
Técnicas de inmunohistoquímica
Streptavidin (SA) is a biotin-binding protein isolated from Streptomyces avidinii, and is similar in size and affinity for biotin. In contrast to avidin, though, streptavidin is not glycosylated, which makes the protein less prone to nonspecific binding in IHC applications.
https://www.thermofisher.com/ar/es/home/life-science/protein...
Replicate tissue sections were assayed for viral antigen by using either a ***streptavidin alkaline phosphatase*** immunohistochemical technique or an immunoperoxidase method with a commercially available kit.
[…] Also note the sharp demarcation between unaffected and affected enanthematous mucosa, the presence of localized staining in the exudate that may represent exfoliated epithelial cells, and that the large intracytoplasmic A-type inclusions do not stain (short arrow)*** (streptavidin alkaline phosphatase [SAAP]***, original magnification ×60)
https://meridian.allenpress.com/aplm/article/124/3/362/61823...
Luego se adicionó el anticuerpo secundario biotinilado (Vector Lab. Cat. No. BA2000), durante 1 hora, seguidode incubación con ***estreptavidina-fosfatasa alcalina*** (Bethesda Research Laboratories, Cat. No. 4542SA) por una hora.
Biomédica Vol 13, Nol-1993 COMUNICACIONES BREVES EVALUACION DE UN METODO INMUNOHlSTOQUlMlCO PARA EL DIAGNOSTICO DE LA FIEBRE AMARILLA

Después de tres lavados con agua destilada los cortes se cubrieron con 2% de albumina bovina y se lavaron repetidamente con tampón fosfato salino. La presencia de compuestos biotinilados se demostró con ***estreptavidina-fosfatasa alcalina*** empleando un equipo comercial (Biogenex).
https://www.sac.org.ar/wp-content/uploads/2014/04/964.pdf

***El polímero de AP*** en dos pasos reconoce las inmunoglobulinas de ratón y conejo y detecta cualquier anticuerpo primario enlazado al tejido. Las secciones se incuban adicionalmente con el substrato/cromógeno, rojo. La reacción con la ***fosfatasa alcalina*** genera un precipitado rojo visible en la posición del antígeno
https://www.menarinidiagnostics.com/Portals/20/pdf_prodotti/...
Selected response from:

María Patricia Arce
Argentina
Local time: 00:08
Grading comment
Gracias, María.
3 KudoZ points were awarded for this answer



Summary of answers provided
4incubación del polímero con estreptavidina-fosfatasa alcalina o FA
María Patricia Arce


  

Answers


1 day 5 hrs   confidence: Answerer confidence 4/5Answerer confidence 4/5
sa‐ap or ap‐polymer incubation
incubación del polímero con estreptavidina-fosfatasa alcalina o FA


Explanation:
Técnicas de inmunohistoquímica
Streptavidin (SA) is a biotin-binding protein isolated from Streptomyces avidinii, and is similar in size and affinity for biotin. In contrast to avidin, though, streptavidin is not glycosylated, which makes the protein less prone to nonspecific binding in IHC applications.
https://www.thermofisher.com/ar/es/home/life-science/protein...
Replicate tissue sections were assayed for viral antigen by using either a ***streptavidin alkaline phosphatase*** immunohistochemical technique or an immunoperoxidase method with a commercially available kit.
[…] Also note the sharp demarcation between unaffected and affected enanthematous mucosa, the presence of localized staining in the exudate that may represent exfoliated epithelial cells, and that the large intracytoplasmic A-type inclusions do not stain (short arrow)*** (streptavidin alkaline phosphatase [SAAP]***, original magnification ×60)
https://meridian.allenpress.com/aplm/article/124/3/362/61823...
Luego se adicionó el anticuerpo secundario biotinilado (Vector Lab. Cat. No. BA2000), durante 1 hora, seguidode incubación con ***estreptavidina-fosfatasa alcalina*** (Bethesda Research Laboratories, Cat. No. 4542SA) por una hora.
Biomédica Vol 13, Nol-1993 COMUNICACIONES BREVES EVALUACION DE UN METODO INMUNOHlSTOQUlMlCO PARA EL DIAGNOSTICO DE LA FIEBRE AMARILLA

Después de tres lavados con agua destilada los cortes se cubrieron con 2% de albumina bovina y se lavaron repetidamente con tampón fosfato salino. La presencia de compuestos biotinilados se demostró con ***estreptavidina-fosfatasa alcalina*** empleando un equipo comercial (Biogenex).
https://www.sac.org.ar/wp-content/uploads/2014/04/964.pdf

***El polímero de AP*** en dos pasos reconoce las inmunoglobulinas de ratón y conejo y detecta cualquier anticuerpo primario enlazado al tejido. Las secciones se incuban adicionalmente con el substrato/cromógeno, rojo. La reacción con la ***fosfatasa alcalina*** genera un precipitado rojo visible en la posición del antígeno
https://www.menarinidiagnostics.com/Portals/20/pdf_prodotti/...


María Patricia Arce
Argentina
Local time: 00:08
Native speaker of: Native in SpanishSpanish
PRO pts in category: 7
Grading comment
Gracias, María.
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