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overlay agar

English translation: plating (out) agar, top agar; soft, low setting temperature agar

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GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
English term or phrase:overlay agar
English translation:plating (out) agar, top agar; soft, low setting temperature agar
Entered by: Jörgen Slet
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09:12 Nov 5, 2003
English to English translations [PRO]
Science / molecular biology
English term or phrase: overlay agar
the direct incorporation method in which bacteria and test agent are mixed in overlay agar and poured over the surface of a selective agar plate.
Jörgen Slet
Estonia
Local time: 01:28
overlay or plating agar
Explanation:
The bacteria and other agents (viruses/phages etc.) are mixed with warm, soft, low setting temperature agar and poured onto preset hard agar plates.

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Note added at 2003-11-05 09:26:43 (GMT)
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The selctive agar plate is set agar with the selective materials already incorporated (usually drugs or antibiotics). The bacteria and test agent are then mixed, preincubated (in order to allow them to be absorbed by the bacteria) and then added to a few ml of melted overlay or plating agar. This is a relatively low melting point agar, and is usually more dilute than the selective agar. This is then poured over the selective plate and allowed to set. The bacteria can then grow in this overlay agar, the less rigid consistency allows relatively free diffusion of gasses and nutrients etc. The idea is that over time, a \"lawn\" of bacteria will form, which may be more or less dense with respect to bacterial growth depending on the effect of the test agent. If the test agent is a lytic phage or virus, this will cause lysis of the bacteria resulting in a clear patch, easily seen against the lawn of \"healthy\" bacteria. This is ised to \"titre\" a phage/virus stock and is also how recombinant cDNA libraries are screened.

Sinonyms are:-
overlay agar
plating (out) agar
top agar etc.

HTH

Sandy

see
http://fruitfly4.aecom.yu.edu/labmanual/61.html

--------------------------------------------------
Note added at 2003-11-05 09:27:43 (GMT)
--------------------------------------------------

sorry - sinonyms for sinonym are synonym!!!
Selected response from:

Alexander Chisholm
Local time: 00:28
Grading comment
Thanks everybody
4 KudoZ points were awarded for this answer

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Summary of answers provided
5 +2overlay or plating agar
Alexander Chisholm


Discussion entries: 2





  

Answers


4 mins   confidence: Answerer confidence 5/5 peer agreement (net): +2
overlay or plating agar


Explanation:
The bacteria and other agents (viruses/phages etc.) are mixed with warm, soft, low setting temperature agar and poured onto preset hard agar plates.

--------------------------------------------------
Note added at 2003-11-05 09:26:43 (GMT)
--------------------------------------------------

The selctive agar plate is set agar with the selective materials already incorporated (usually drugs or antibiotics). The bacteria and test agent are then mixed, preincubated (in order to allow them to be absorbed by the bacteria) and then added to a few ml of melted overlay or plating agar. This is a relatively low melting point agar, and is usually more dilute than the selective agar. This is then poured over the selective plate and allowed to set. The bacteria can then grow in this overlay agar, the less rigid consistency allows relatively free diffusion of gasses and nutrients etc. The idea is that over time, a \"lawn\" of bacteria will form, which may be more or less dense with respect to bacterial growth depending on the effect of the test agent. If the test agent is a lytic phage or virus, this will cause lysis of the bacteria resulting in a clear patch, easily seen against the lawn of \"healthy\" bacteria. This is ised to \"titre\" a phage/virus stock and is also how recombinant cDNA libraries are screened.

Sinonyms are:-
overlay agar
plating (out) agar
top agar etc.

HTH

Sandy

see
http://fruitfly4.aecom.yu.edu/labmanual/61.html

--------------------------------------------------
Note added at 2003-11-05 09:27:43 (GMT)
--------------------------------------------------

sorry - sinonyms for sinonym are synonym!!!

Alexander Chisholm
Local time: 00:28
Native speaker of: English
PRO pts in pair: 74
Grading comment
Thanks everybody

Peer comments on this answer (and responses from the answerer)
agree  David Sirett
16 mins

agree  Anna Haxen
1 hr
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