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15:55 Sep 23, 2007
English to German translations [PRO] Science - Genetics
English term or phrase:abundance
To assess the tissue distribution of AB014553 cDNA and AB014553 RACE clones, RT-PCR/southern blot analyses were performed under similar conditions as for the GL50 sequences described above.Using oligonucleotides primers specific for the amplification of published AB014553 cDNA (VL142 (ACAACAGCCTGCTGGACCAGGC; SEQ ID NO:10) and VL163B (GGGCCCCCCAGAACCTGCTGCTTCC; SEQ ID NO:17)), PCR resulted in the complete absence of any detectable AB014553 cDNA signal for all samples tested (Figure 10).Possible explanations for the lack of RT-PCR products representing published AB014553 cDNA sequences may be the use of non-optimized oligonucleotides, extremely low ***abundance*** of the target transcript, or actual absence of this form of the product.