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English to Polish translations [PRO] Science - Biology (-tech,-chem,micro-) / Enzymes
English term or phrase:N-terminal Leu
Degradation with cyanogen bromide of thrombin produced 6 fragments from the B chain and the intact A chain.
Good separation was obtained in 2 steps on a Sephadex column in 20 mM acetic acid. In the first step the digest was separated. 4 fractions were obtained. The first one contained the A chain; and the B chain had N-terminal Leu-, Thr- and Phe-.