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  3. Biology (-tech,-chem,micro-)

recombinant kinase domain of the ALK

Polish translation: rekombinowana domena ALK o aktywności kinazy

21:18 Sep 5, 2008
English to Polish translations [PRO]
Science - Biology (-tech,-chem,micro-)
English term or phrase: recombinant kinase domain of the ALK
The efficacy of the compounds of formula (I) as inhibitors of ALK tyrosine kinase activity can be demonstrated as follows: The inhibition of ALK tyrosine kinase activity can be demonstrated using known methods, e.g., using the recombinant kinase domain of the ALK in analogy to the VEGF-R kinase assay. See Wood et al., Cancer Res, Vol. 60, No. 8, pp. 2178-2189 (2000). In vitro enzyme assays using GST-ALK protein tyrosine kinase are performed in 96-well plates as a filter binding assay in 20 mM tris·HCl, pH=7.5, 3 mM MgCl2, 10 mM MnCl2, 1 mM DTT, 0.1 PCi/assay (=30
PL) [g-33P]-ATP, 2 PM ATP, 3 Pg/mL poly (Glu, Tyr 4:1) Poly-EY (Sigma P-0275), 1% DMSO, 25 ng ALK enzyme. Assays are incubated for 10 minutes at ambient temperature. Reactions are terminated by adding 50 PL of 125 mM EDTA, and the reaction mixture is transferred onto a MAIP Multiscreen plate (Millipore, Bedford, MA, USA), previously wet with methanol, and re-hydrated for 5 minutes with H2O. Following washing (0.5% H3PO4), plates are counted in a
liquid scintillation counter. IC50 values are calculated by linear regression analysis of the percentage inhibition. Compared with the control without inhibitor, the compounds of formula (I) inhibit the enzyme activity by 50% (IC50), e.g., in a concentration of from 0.001-0.5 PM, especially from 0.1-0.8 PM.
Barbara Piela
Local time: 10:55
Polish translation:rekombinowana domena ALK o aktywności kinazy
Explanation:
wolę tego typu zwrot, choć domena kinazowa ujdzie
Selected response from:

Piotr Sawiec
Local time: 10:55
Grading comment
Dziękuję!
4 KudoZ points were awarded for this answer



Summary of answers provided
4rekombinowana domena ALK o aktywności kinazy
Piotr Sawiec
3rekombinowana domena kinazowa ALK (anaplastycznej kinazy chłoniaka)
kereish


  

Answers


9 mins   confidence: Answerer confidence 3/5Answerer confidence 3/5
recombinant kinase domain of the alk
rekombinowana domena kinazowa ALK (anaplastycznej kinazy chłoniaka)


Explanation:
http://bioinfo.mol.uj.edu.pl/articles/Nielipinska06

kereish
Local time: 10:55
Specializes in field
Native speaker of: Native in PolishPolish
PRO pts in category: 15
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1 day 20 hrs   confidence: Answerer confidence 4/5Answerer confidence 4/5
recombinant kinase domain of the alk
rekombinowana domena ALK o aktywności kinazy


Explanation:
wolę tego typu zwrot, choć domena kinazowa ujdzie

Piotr Sawiec
Local time: 10:55
Native speaker of: Native in PolishPolish
PRO pts in category: 88
Grading comment
Dziękuję!
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