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pour off

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20:44 Mar 22, 2018
English to Polish translations [PRO]
Science - Environment & Ecology / general
English term or phrase: pour off
Z tabeli NPL:
MPN is per ml of the 1 mL added to 9 mL rehydrated media (pour off is accounted for).
Małgorzata Rymsza
United Kingdom
Local time: 23:45

Summary of answers provided
2odprowadzenie nadmiaru cieczy (z góry naczynia/zbiornika)
Frank Szmulowicz, Ph. D.
Summary of reference entries provided
Test Procedure

Discussion entries: 3



3 hrs   confidence: Answerer confidence 2/5Answerer confidence 2/5
odprowadzenie nadmiaru cieczy (z góry naczynia/zbiornika)

to drain liquid off the top of something
https://idioms.thefreedictionary.com/pour off

Frank Szmulowicz, Ph. D.
United States
Local time: 18:45
Native speaker of: Native in EnglishEnglish, Native in PolishPolish
PRO pts in category: 77
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6 hrs   confidence: Answerer confidence 3/5Answerer confidence 3/5


1. Przygotować się do pracy pod komorą laminarną (patrz: ćwiczenie „Hodowle komórkowe”)
2. Wykonać pasaż komórek:
a) z naczynia hodowlanego zlać pożywkę
b) przepłukać komórki 1 ml PBSu
c) po zlaniu PBSu dodać do komórek 1 ml trypsyny i inkubować ok. 10 sekund
d) zlać nadmiar roztworu enzymu, pozostawiając jedynie jego niewielką ilość i umieścić naczynie w cieplarce na ok. 2-3 minuty, kontrolując („pod światło”) co jakiś czas stopień odrywania się komórek od podłoża

http://www.uj.edu.pl/documents/2387936/4117782/cw7bc.pdf - page 3

Native speaker of: Native in PolishPolish
PRO pts in category: 51
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Reference comments

6 hrs
Reference: Test Procedure

Reference information:
1. Hydrate media by filling media vessel to the 100 mL mark with sterile diluent (e.g., dechlorinated water, deionized water, phosphate buffer, or 0.1% peptone), recapping and shaking to dissolve. See image #1.

2. Pipette 1 mL sample and then 9 mL of the rehydrated media onto the center of the plate base. See image #2.

3. Cover the plate with the lid and gently swirl to distribute the sample into all the wells. See image #3. NOTE: Air bubbles in the wells do not interfere with the test.

4. Tip the plate 90–120° to drain excess sample into the absorbent pad. See image #4.

5. Invert the plate and incubate for 48 hours at 35±0.5°C. See image #5.

6. Count the number of wells showing any fluorescence by holding a 6-watt, 365-nm UV light 5 inches above the plate. Direct light away from your eyes and towards the sample. Alternatively, you may read
fluorescent wells through the back of the inverted SimPlate base.

7. Refer to the MPN table provided to determine the Most Probable Number of heterotrophic plate count bacteria in the original sample. The table takes into account the sample/media poured off in step #4 above.


Native speaker of: Native in PolishPolish
PRO pts in category: 51
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