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plating efficiency

Polish translation: wydajność procesu posiewania bakterii na pożywce stałej

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23:46 Nov 2, 2003
English to Polish translations [PRO]
Science
English term or phrase: plating efficiency
XXX is a reducing agent which has been shown to enhance growth, plating efficiency and/or antibody synthesis of specific cell lines.
Romuald Pawlikowski
Local time: 06:10
Polish translation:wydajność procesu posiewania bakterii na pożywce stałej
Explanation:
Slomscy:
plating - posiewanie bakterii na pożywce stałej

Tu opisuja cos takiego:
http://iai.asm.org/cgi/content/full/66/9/4440

Monitoring of C. albicans in experimental skin infections. After establishing a skin infection with C. albicans or PBS (sham control), occlusive dressings were removed, the mice were sacrificed, and skin biopsy specimens were taken from the infection site at selected times. The skin biopsy specimens were weighed on a Mettler triple beam balance to the nearest 0.01 g and were then transferred to a sterile microfuge tube. To each tube, 400 µl of tissue culture grade 0.25% trypsin-EDTA solution (Gibco/BRL, Gaithersburg, Md.) was added. The tubes were incubated at 37°C for 30 min with intermittent vortexing. Pilot studies indicated that this enzymatic solution did not affect the viability or the colony-forming ability of C. albicans (which had 100% plating efficiency after exposure to 0.25% trypsin-EDTA). Serial dilutions of the dissociated tissue slurry were plated on YEPD agar plates, the plates were incubated for 48 h at 37°C, and colony forming units (CFU) were counted. Data on the total CFU were divided by the tissue weight (in grams), resulting in values for CFU/gram of tissue. The CFU/gram of tissue values for each of the mice in an experimental group were pooled together and expressed as a mean ± standard error of the mean (SEM). A semiquantitative analysis of the presence of C. albicans in mice allowed to recover from infection by the removal of the occlusive dressing was performed by swabbing the previously infected area with a cotton swab dipped in sterile PBS and rolling the swab onto YEPD plates. A relative evaluation of organism numbers was performed by assessing the C. albicans CFU obtained in this manner.
Selected response from:

Barbara Piela
Local time: 06:10
Grading comment
Dziekuje
4 KudoZ points were awarded for this answer

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Summary of answers provided
3 +2wydajność procesu posiewania bakterii na pożywce stałej
Barbara Piela


  

Answers


1 hr   confidence: Answerer confidence 3/5Answerer confidence 3/5 peer agreement (net): +2
wydajność procesu posiewania bakterii na pożywce stałej


Explanation:
Slomscy:
plating - posiewanie bakterii na pożywce stałej

Tu opisuja cos takiego:
http://iai.asm.org/cgi/content/full/66/9/4440

Monitoring of C. albicans in experimental skin infections. After establishing a skin infection with C. albicans or PBS (sham control), occlusive dressings were removed, the mice were sacrificed, and skin biopsy specimens were taken from the infection site at selected times. The skin biopsy specimens were weighed on a Mettler triple beam balance to the nearest 0.01 g and were then transferred to a sterile microfuge tube. To each tube, 400 µl of tissue culture grade 0.25% trypsin-EDTA solution (Gibco/BRL, Gaithersburg, Md.) was added. The tubes were incubated at 37°C for 30 min with intermittent vortexing. Pilot studies indicated that this enzymatic solution did not affect the viability or the colony-forming ability of C. albicans (which had 100% plating efficiency after exposure to 0.25% trypsin-EDTA). Serial dilutions of the dissociated tissue slurry were plated on YEPD agar plates, the plates were incubated for 48 h at 37°C, and colony forming units (CFU) were counted. Data on the total CFU were divided by the tissue weight (in grams), resulting in values for CFU/gram of tissue. The CFU/gram of tissue values for each of the mice in an experimental group were pooled together and expressed as a mean ± standard error of the mean (SEM). A semiquantitative analysis of the presence of C. albicans in mice allowed to recover from infection by the removal of the occlusive dressing was performed by swabbing the previously infected area with a cotton swab dipped in sterile PBS and rolling the swab onto YEPD plates. A relative evaluation of organism numbers was performed by assessing the C. albicans CFU obtained in this manner.



    Reference: http://iai.asm.org/cgi/content/full/66/9/4440
Barbara Piela
Local time: 06:10
Native speaker of: Native in PolishPolish
PRO pts in pair: 830
Grading comment
Dziekuje

Peer comments on this answer (and responses from the answerer)
agree  Agnieszka Hayward: tako rzecze medyczny słownik
2 mins
  -> ;-)

agree  Stefan Simko
6 hrs
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