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double stranded primer-extended templates

Russian translation: двухцепочечная матрица в комплексе с праймером

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15:54 Nov 22, 2013
English to Russian translations [PRO]
Science - Biology (-tech,-chem,micro-)
English term or phrase: double stranded primer-extended templates
Речь идет о PE-PCR (primer-extension PCR ).

For example, microbial DNA may be extracted from sample reservoir 3 into reaction chambers 4 and 5 whereto the fusion probes and EK mix may be added so as to form ""primer-extended templates. The primer-extended templates may then be amplified by PCR in PCR chamber 6. The PCR reaction chamber may then be forwarded to an sample analyzing unit 7

Эпап ПЦР:

extending the З'-ends of the fusion probes and the microbial DNA to form double stranded primer-extended templates


The strand that extended from the fusion probe will have the non-bacterial tagging sequence located at its 5'-end whereas the strand that extended from the target DNA template will have the non-bacterial tagging sequence located at its З'-end.

Для "extended" ранее был предложен термин "амплифицированные", но он не подходит.

Заранее огромное спасибо!
dao2812
Russian translation:двухцепочечная матрица в комплексе с праймером
Explanation:
Чтобы было понятнее, посмотрите вот здесь http://web-local.rudn.ru/web-local/prep/rj/files.php?f=pdf_1... рисунок на стр. 2 в разделе "Полимеризация или элонгация": то, о чем идет речь здесь, выглядит примерно как последний этап на этом рисунке.
Selected response from:

Natalie
Poland
Local time: 18:47
Grading comment
Спасибо огромное!!!
4 KudoZ points were awarded for this answer

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Summary of answers provided
4двухцепочечная матрица в комплексе с праймером
Natalie


  

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3 hrs   confidence: Answerer confidence 4/5Answerer confidence 4/5
двухцепочечная матрица в комплексе с праймером


Explanation:
Чтобы было понятнее, посмотрите вот здесь http://web-local.rudn.ru/web-local/prep/rj/files.php?f=pdf_1... рисунок на стр. 2 в разделе "Полимеризация или элонгация": то, о чем идет речь здесь, выглядит примерно как последний этап на этом рисунке.

Natalie
Poland
Local time: 18:47
Specializes in field
Native speaker of: Native in RussianRussian
PRO pts in category: 3921
Grading comment
Спасибо огромное!!!
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