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|English to Russian translations [PRO]|
Science - Biology (-tech,-chem,micro-) / micribiology
|English term or phrase: pre-blocked|
|Standard histology was performed on the BCC cultures and slides were de-paraffinized, pre-blocked and incubated with primary antibody|
...срезы были депарафинированы, обработаны блокирующим раствором и проинкубированы с первичными антителами
pre- в данном случае означает пред-, предварительно, однако в русском варианте это не имеет никакого особого значения, и я бы обошлась без этой приставки
Selected response from:
Local time: 01:15
|Спасибо, Натали ! Спасибо, Мyrafla ! |
4 KudoZ points were awarded for this answer
31 mins confidence:
Kultury byli podverzheny standartnomu gistologicheskomu analizu, a slaidy byli ochischeny ot parafina, obrabotany predblochnym rastvorom i inkubirovany nachalnym antitelom.
...followed by one 5-min wash with ddH2O; preblock buffer (0.05% Triton-X, 5% goat serum in PBS) at 4°C for 1.5 h; primary antibodies in preblock buffer at 4°C overnight. After primary antibody incubation, the sections were washed for 30 min three times with preblock buffer and then incubated with FITC-conjugated secondary antibodies (Jackson ImmunoResearch; dilution, 1:200) at room temperature for 1 h followed by one 3-min wash with preblock buffer and two 30-min washes with PBS. Sections were mounted in Immuno Floure Mounting
Local time: 19:15
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Native speaker of: Russian
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