pGEM control plasmid template

Russian translation: контрольная ДНК матрицы плазмиды pGEM

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15:08 Jul 8, 2018
English to Russian translations [PRO]
Chemistry; Chem Sci/Eng / PCR Assay
English term or phrase: pGEM control plasmid template
B. Setting up sequence reaction.
a) Applied BioSystems Big Dye Terminator Sequencing Kit is used on this step.
b) Label a vial for each sample to be sequenced – 2 vials for each sample to be sequenced – one with forward primer and one with reverse primer.
c) Prepare the sequencing reaction as follows:
• 1 to 3 μl of purified DNA template (PCR product from a previous step after we examined the gel picture and decided on a quantity).
• 2 μl of Big Dye Terminator Mix (aliquots are in -20oC freezer)
• 1 μl of 2 μM Primer (Dilute PCR 20 μM stock 1:10 in dist. water)
• 1 μl of 5x Sequence Buffer (aliquots are in 4°C refrigerator)
• dH20 (to make final volume 10 l)
d) Prepare one control sequence reaction as follows:
• 1.5 μl of pGEM control plasmid template (Applied BioSystems)
• 2 μl of Big Dye Terminator Mix
• 4 μl of M13 primer (Applied BioSystems)
arinaotm
Russian translation:контрольная ДНК матрицы плазмиды pGEM
Explanation:
*

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Note added at 2 hrs (2018-07-08 17:51:00 GMT)
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Вот пример, кстати:

Средства поверки:
Поверка проводится с использованием контрольной ДНК матрицы плазмиды pGEM-Zf(+) с известной нуклеотидной последовательностью, размером в 1000 нуклеотидов. В качестве контрольного инициирующего праймера для секвенирования в этом случае применяют праймер -21 М13 (forward).
https://ib.komisc.ru/add/molbiol/?page_id=6
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Natalie
Poland
Local time: 12:35
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Summary of answers provided
4 +1контрольная ДНК матрицы плазмиды pGEM
Natalie


  

Answers


33 mins   confidence: Answerer confidence 4/5Answerer confidence 4/5 peer agreement (net): +1
pgem control plasmid template
контрольная ДНК матрицы плазмиды pGEM


Explanation:
*

--------------------------------------------------
Note added at 2 hrs (2018-07-08 17:51:00 GMT)
--------------------------------------------------

Вот пример, кстати:

Средства поверки:
Поверка проводится с использованием контрольной ДНК матрицы плазмиды pGEM-Zf(+) с известной нуклеотидной последовательностью, размером в 1000 нуклеотидов. В качестве контрольного инициирующего праймера для секвенирования в этом случае применяют праймер -21 М13 (forward).
https://ib.komisc.ru/add/molbiol/?page_id=6

Natalie
Poland
Local time: 12:35
Specializes in field
Native speaker of: Native in RussianRussian
PRO pts in category: 1414

Peer comments on this answer (and responses from the answerer)
agree  svetlana cosquéric
3 hrs
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