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|English to Russian translations [PRO]|
Medical - Medical (general)
|English term or phrase: hemagglutinin "HA" tag|
|The present invention also encompasses antibodies or antigen-binding fragments thereof that preferentially bind to cell-associated CA 125/O772P fused to marker sequences, such as a peptide to facilitate purification. For example, a marker amino acid sequence can be a hexa-histidine peptide, such as the tag provided in a pQE vector (QIAGEN, Inc., 9259 Eton Avenue, Chatsworth, CA, 91311), among others, many of which are commercially available. As described in Gentz et al., Proc. Natl. Acad. Sci. USA. 86(3):821-824 (1989), for instance, histidine, e.g., hexa-histidine, provides for convenient purification of the fusion protein. Other peptide tags useful for purification include, but are not limited to, the ***hemagglutinin "HA" tag***, which corresponds to an epitope derived from the influenza hemagglutinin protein (Wilson et al., Cell. 37(3):767-778 (1984)) and the ***"flag" tag*** (Brizzard et al., Biotechniques. 16(4):730-735 (1994)). Preferably, such ***tags or marker sequences*** are cleaved from the fusion polypeptide prior to use, e.g., use as part of a therapeutic method.|
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