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Antigen enhancement

Russian translation: усиление антигенности

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14:50 Nov 12, 2008
English to Russian translations [PRO]
Medical - Medical (general) / Histology
English term or phrase: Antigen enhancement
Antigen enhancement (cell conditioning) procedure (for tissue slides to be stained on .....

This negative tissue control should be stained with.... to ensure that the antigen enhancement and other pretreatment procedures did not create false positive staining
Cherepanov
Ukraine
Local time: 05:19
Russian translation:усиление антигенности
Explanation:
Речь идет о видоизменении вещества в ходе лабораторной обработки реактивами с последующим усилением антигенных свойств, что может приводить к ложноположительным результатам

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Note added at 8 mins (2008-11-12 14:58:49 GMT)
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http://cat.inist.fr/?aModele=afficheN&cpsidt=3041982
Immunohistochemistry (IHC) for intranuclear p53 gene product is a simple, routine alternative to molecular genetic analysis of the p53 gene. Several methods for antigen enhancement are currently in use for IHC. This study evaluates the effect of extreme antigen enhancement for p53, using a monoclonal antibody (DO7) and a polyclonal antibody (CM1). The cases studied were five colorectal carcinomas, two specimens of normal colorectal mucosa, and four colorectal carcinomas with genetic alterations which are expected to preclude p53 gene product expression, namely mutation to a STOP codon in the p53 gene detected by denaturing gradient gel electrophoresis with subsequent sequencing and allelic loss of 17p in the region where p53 is located, detected by restriction fragment length polymorphism analysis. The findings suggest that extreme antigen enhancement may cause false-positive results with a distinct nuclear staining pattern when MAb DO7 is used as a primary antibody. It is concluded that all antigen enhancement methods should be thoroughly tested to evaluate their validity and that there may be a limit to the extent to which antigen enhancement can be applied in IHC for p53 protein.
Selected response from:

Pavel Nikonorkin
Local time: 05:19
Grading comment
Спасибо!
4 KudoZ points were awarded for this answer

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Summary of answers provided
4 +2усиление антигенности
Pavel Nikonorkin


  

Answers


8 mins   confidence: Answerer confidence 4/5Answerer confidence 4/5 peer agreement (net): +2
antigen enhancement
усиление антигенности


Explanation:
Речь идет о видоизменении вещества в ходе лабораторной обработки реактивами с последующим усилением антигенных свойств, что может приводить к ложноположительным результатам

--------------------------------------------------
Note added at 8 mins (2008-11-12 14:58:49 GMT)
--------------------------------------------------

http://cat.inist.fr/?aModele=afficheN&cpsidt=3041982
Immunohistochemistry (IHC) for intranuclear p53 gene product is a simple, routine alternative to molecular genetic analysis of the p53 gene. Several methods for antigen enhancement are currently in use for IHC. This study evaluates the effect of extreme antigen enhancement for p53, using a monoclonal antibody (DO7) and a polyclonal antibody (CM1). The cases studied were five colorectal carcinomas, two specimens of normal colorectal mucosa, and four colorectal carcinomas with genetic alterations which are expected to preclude p53 gene product expression, namely mutation to a STOP codon in the p53 gene detected by denaturing gradient gel electrophoresis with subsequent sequencing and allelic loss of 17p in the region where p53 is located, detected by restriction fragment length polymorphism analysis. The findings suggest that extreme antigen enhancement may cause false-positive results with a distinct nuclear staining pattern when MAb DO7 is used as a primary antibody. It is concluded that all antigen enhancement methods should be thoroughly tested to evaluate their validity and that there may be a limit to the extent to which antigen enhancement can be applied in IHC for p53 protein.

Pavel Nikonorkin
Local time: 05:19
Specializes in field
Native speaker of: Native in RussianRussian
PRO pts in category: 1479
Grading comment
Спасибо!

Peer comments on this answer (and responses from the answerer)
agree  Ol_Besh
2 hrs
  -> Спасибо, Олександр!

agree  Natalie79
16 hrs
  -> Спасибо, Натали!
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