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Activity by displacement of HABA by biotin

Russian translation: активность по вытеснению HABA биотином

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13:14 Nov 15, 2008
English to Russian translations [PRO]
Medical - Medical: Health Care
English term or phrase: Activity by displacement of HABA by biotin
Assay: Activity by displacement of HABA
by biotin
esperansa_2008
Russian translation:активность по вытеснению HABA биотином
Explanation:
не очень понятно, при чем тут активность...

2-(4'-hydroxyazobenzene)-2-carboxylic acid (HABA)
2-(4'-гидроксиазобензил)-2-карбоновая кислота

метод служит для определения количества биотиновых остатков в молекуле белка

Researchers estimate the extent of biotinylation to optimize a particular avidin:biotin-based assay system and to ensure reproducibility in the biotinylation process. 2-(4'-hydroxyazobenzene)-2-carboxylic acid (HABA) binds weakly to avidin and absorbs characteristically at 500 nm. It can be displaced from its avidin-binding sites by biotin.

The HABA displacement method relies on the decrease in absorbance of the HABA:avidin complex upon displacement of the HABA by biotin from the labeled protein. The number of moles of biotin per mole of protein can be estimated by this method.

http://www.piercenet.com/Objects/View.cfm?type=ProductFamily...

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Note added at 23 mins (2008-11-15 13:37:55 GMT)
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нужно больше контекста, чтобы понять о чем речь. Активность чего изучают?
Selected response from:

mas63
Russian Federation
Local time: 02:11
Grading comment
1 KudoZ points were awarded for this answer

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Summary of answers provided
4активность по вытеснению HABA биотином
mas63


  

Answers


18 mins   confidence: Answerer confidence 4/5Answerer confidence 4/5
activity by displacement of haba by biotin
активность по вытеснению HABA биотином


Explanation:
не очень понятно, при чем тут активность...

2-(4'-hydroxyazobenzene)-2-carboxylic acid (HABA)
2-(4'-гидроксиазобензил)-2-карбоновая кислота

метод служит для определения количества биотиновых остатков в молекуле белка

Researchers estimate the extent of biotinylation to optimize a particular avidin:biotin-based assay system and to ensure reproducibility in the biotinylation process. 2-(4'-hydroxyazobenzene)-2-carboxylic acid (HABA) binds weakly to avidin and absorbs characteristically at 500 nm. It can be displaced from its avidin-binding sites by biotin.

The HABA displacement method relies on the decrease in absorbance of the HABA:avidin complex upon displacement of the HABA by biotin from the labeled protein. The number of moles of biotin per mole of protein can be estimated by this method.

http://www.piercenet.com/Objects/View.cfm?type=ProductFamily...

--------------------------------------------------
Note added at 23 mins (2008-11-15 13:37:55 GMT)
--------------------------------------------------

нужно больше контекста, чтобы понять о чем речь. Активность чего изучают?

mas63
Russian Federation
Local time: 02:11
Works in field
Native speaker of: Native in RussianRussian
PRO pts in category: 119
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