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English to Spanish translations [PRO] Chemistry; Chem Sci/Eng / gradiente de densidad
English term or phrase:8 vol. (ver contexto)
Dilution of 100% XXFilter®
Pre-made ready-to-use 40 % and 80 % gradients are available from Cryos. However, gradients of individual choices can be prepared.
Mix the 100% XXFilter® solution carefully before use.
To make an 80 % XXFilter® gradient dilute 8 vol. of XXFilter® with 2 vol. culture medium. After carefully mixing the 80% gradient take 1 vol. 80 % XXilter® gradient and dilute with 1 vol. culture medium.
To prepare 8 ml 80% and 8 ml. 40% XXFilter® gradients:
80% XXFilter® gradient: Take 9.6 ml 100% XXFilter® and 2.4 ml culture medium. Carefully mix this 80% gradient.
40% XXFilter® gradient: Take 4.0 ml of the 80% gradient and dilute with 4.0 ml. culture medium.