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raised against


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20:00 Jan 18, 2008
This question was closed without grading. Reason: Errant question

English to Spanish translations [PRO]
Science - Medical: Pharmaceuticals / well cut
English term or phrase: raised against
Para determinar el contenido de antígeno de hemaglutinina mediante SRID, se incorporan en agarosa anticuerpos del antígeno de hemaglutinina específico de la cepa de la vacuna sobre la cual se está realizando la prueba, se vuelcan en una placa de vidrio y se dejan asentar. Las muestras de la vacuna contra la influenza se fraccionan con detergente para solubilizar el antígeno de hemaglutinina. Se deja que las diluciones de las muestras fraccionadas se disperse mediante la agarosa "from a well cut" en la azarosa asentada. Luego los complejos de antígenos y anticuerpos se tiñen y se mide la superficie de las zonas elípticas o circulares del complejo teñido (mm2). La superficie de una zona es proporcional a la cantidad de antígeno (hemaglutinina) en la muestra según se compara con un antígeno estándar.
xxxPaula Gonzal
Local time: 18:55

Summary of answers provided
4pocillo vaciado
liz askew

Discussion entries: 3



6 mins   confidence: Answerer confidence 3/5Answerer confidence 3/5

Your query should be "well"...

n the course of gene and genome manipulation, it is often necessary to detect and isolate specific DNA molecules from a mixture. For example, recall that, in cloning with the use of l phage, it is necessary to separate the two chromosome arms from the unwanted central region. There are several ways of fractionating DNA, but the most extensively used method is electrophoresis (see Figure 6-5 for a drawing of an electrophoretic apparatus). If a mixture of linear DNA molecules is placed in a well cut into an agarose gel and the well is placed near the cathode of an electric field, the molecules will move through the gel to the anode, at speeds dependent on their size (Figure 10-17). Therefore, if there are distinct size classes in the mixture, these classes will form distinct bands on the gel. The bands can be visualized by staining the DNA with ethidium bromide, which causes the DNA to fluoresce in UV light. If bands are well separated, an individual band can be cut from the gel and the purified DNA sample can be removed from the gel matrix. Therefore DNA electrophoresis can be either diagnostic (showing which DNA fragments are present) or preparative (useful in isolating specific DNA fragments).

Gel de agarosa
File Format: PDF/Adobe Acrobat - View as HTML
previamente. Colocar la micropipeta en forma perpendicular al gel de. agarosa, e introducirla hasta el fondo del pozo liberando su contenido ...
www.medvet.una.ac.cr/carrera/mva505_Practica8.pdf - Similar pages
Genética médica - Google Books Result
by Rafael Oliva Virgili, Rafael Oliva - 2004
El conjunto (Tampón de carga + muestra) de cada amplificación, lo cargaremos en los pozos del gel de agarosa (figura 26.3). ...

Note added at 7 mins (2008-01-18 20:07:34 GMT)

You would do well (!) to put the whole context in ENGLISH, as this would facilitate a decent search of your terminology.

liz askew
United Kingdom
Local time: 21:55
Works in field
Native speaker of: Native in EnglishEnglish
PRO pts in category: 213
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25 mins   confidence: Answerer confidence 4/5Answerer confidence 4/5
pocillo vaciado

Se vacía un pocillo en la agarosa solidificada

Local time: 22:55
Specializes in field
Native speaker of: Native in SpanishSpanish
PRO pts in category: 107
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