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Subject Agriculture - Food Industry - Livestock Industry - Fisheries - Bio-industry (AG)
TERM to pellet
Slide preparation. Swabs in viral transport medium were vortexed, wrung out, and then discarded. To remove mucous from NP aspirates, 5 ml of phosphate-buffered saline (PBS) was added and specimens were pipetted up and down. Samples were then centrifuged at 700 × g for 5 min to pellet cells for DFA. The cell pellets were resuspended in a small amount of PBS, and 200 µl of the suspension was applied per cell spot by cytocentrifugation (Cytospin 3; Shandon Inc., Pittsburgh, Pa.) at 800 rpm for 4 min. Slides were air dried and then fixed in cold acetone for 10 min. For the purpose of the study, two to three cytospin slides were prepared depending on the viruses suspected.