Ukrainian translation: концентрація за даними УФ-поглинання
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English term or phrase:Concentration by UV / UV absorption
(4) Concentration by UV. The protein concentration was determined by UV absorption at 280 nm. A UV/VIS diode array spectrophotometer (HP8253A) was employed with a 1-cm path-length quartz cuvette for measurement. The samples were diluted 10 to 12.5 fold with formulation buffer. The measurement was blanked against the proper reference and an UV scan from 240 to 400 nm was taken. The protein concentration was calculated as follows: