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Mausmilzzellsuspension

English translation: mouse spleen cell suspension

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GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
German term or phrase:Mausmilzzellsuspension
English translation:mouse spleen cell suspension
Entered by: Paula Price
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09:25 Oct 24, 2007
German to English translations [PRO]
Tech/Engineering - Biology (-tech,-chem,micro-)
German term or phrase: Mausmilzzellsuspension
Das Serum wird in verschiedenen Mengen einer Mausmilzzellsuspension in vitro beigemischt.
Paula Price
Local time: 13:59
mouse spleen cell suspension
Explanation:
"Total DCs were isolated from a mouse spleen cell suspension using Pan DC MicroBeads, a MiniMACS™ Separator, and two MS Columns. The cells are fluorescently stained with CD11c-PE (# 130-091-830) and Anti-mPDCA-1-APC (# 130-091-963). Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence."

_________________________________

"In the wells of a 96-well flat-bottomed microplate, 100 µl of C57BL/6 mouse spleen cell suspension, 50 µl of mitomycin-treated Balb/c mouse spleen cell suspension and 50 µl of RPMI 1640 containing the test compound.."

Selected response from:

Nicole Schnell
United States
Local time: 05:59
Grading comment
Thanks a lot!
4 KudoZ points were awarded for this answer

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Summary of answers provided
4 +5mouse spleen cell suspension
Nicole Schnell


  

Answers


13 mins   confidence: Answerer confidence 4/5Answerer confidence 4/5 peer agreement (net): +5
mouse spleen cell suspension


Explanation:
"Total DCs were isolated from a mouse spleen cell suspension using Pan DC MicroBeads, a MiniMACS™ Separator, and two MS Columns. The cells are fluorescently stained with CD11c-PE (# 130-091-830) and Anti-mPDCA-1-APC (# 130-091-963). Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence."

_________________________________

"In the wells of a 96-well flat-bottomed microplate, 100 µl of C57BL/6 mouse spleen cell suspension, 50 µl of mitomycin-treated Balb/c mouse spleen cell suspension and 50 µl of RPMI 1640 containing the test compound.."




    Reference: http://72.14.253.104/search?q=cache:sfoihmk8Hy0J:www.milteny...
    Reference: http://72.14.253.104/search?q=cache:kFiS5vCbdmgJ:www.patents...
Nicole Schnell
United States
Local time: 05:59
Native speaker of: Native in GermanGerman
PRO pts in category: 8
Grading comment
Thanks a lot!

Peer comments on this answer (and responses from the answerer)
agree  Eckhard Boehle
11 mins
  -> Thanks, Eckhard!

agree  Maurice Claypole
42 mins
  -> Thanks, Maurice!

agree  Cetacea: Genau!
2 hrs
  -> Thanks, Christa!

agree  Jeremy Amos
4 hrs
  -> Thanks, Jeremy!

agree  MMUlr: or also (alternatively) -> murine ....
5 hrs
  -> Thanks, MMUlr!
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Changes made by editors
Oct 24, 2007 - Changes made by Steffen Walter:
Field (specific)Chemistry; Chem Sci/Eng » Biology (-tech,-chem,micro-)


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