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Peroxidase-vermittelte Farbstoffumsetzung

English translation: peroxydase induced color change

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07:37 Aug 20, 2001
German to English translations [PRO]
Medical
German term or phrase: Peroxidase-vermittelte Farbstoffumsetzung
Zum Nachweis der anti-p53-Autoantikörper wurde ein Sandwich-ELISA (enzyme linked immuno sorbent assay) der Fa. Dianova, Hamburg mit Festphase-gekoppeltem, rekombinantem p53-Protein verwendet, das die anti-p53-Autoantikörper aus der Serumprobe bindet. Der Nachweis erfolgte über ein Peroxidase-konjugiertes antihumanes IgG (Ziege) als Detektor und die Messung einer Peroxidase-vermittelten Farbstoffumsetzung.
Caro Maucher
Germany
Local time: 16:05
English translation:peroxydase induced color change
Explanation:
vermitteln = to indicate, to put in evidence.
Umsetzung = displacement, transformation
Farbstoff = colorant, pigment

Farbstoffumsetzung, can be interpreted as a colorant migration or a colorant transformation, that will change it's color. I prefer the second hypothesis because peroxydation generally have discoloring effects on organic material. From it's name, a peroxydase is an enzym that will induce such an oxydation, thus induce such a color change.
I think that "vermittelten" is not used exactly in the sense of indicating, but rather in the sense of revealing.
A literal translation would be
"peroxydase indicated colorchange
Selected response from:

Yves Georges
France
Local time: 16:05
Grading comment
Thank you, I think I like your answer best!
4 KudoZ points were awarded for this answer

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Summary of answers provided
na +1peroxydase induced color change
Yves Georges
naperoxidase-mediated color change
Tom Funke
naperoxidase-mediated color conversionUlrike Lieder
naAddendum to my answer:
Sven Petersson
naperoxidase conveyed colour reaction
Mats Wiman
nacolour change produced by peroxidase
Sven Petersson


  

Answers


49 mins
colour change produced by peroxidase


Explanation:
None needed.


    My geriatric brain.
Sven Petersson
Sweden
Local time: 16:05
Native speaker of: Native in SwedishSwedish, Native in EnglishEnglish
PRO pts in pair: 1628
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52 mins
peroxidase conveyed colour reaction


Explanation:
none

Mats Wiman
Sweden
Local time: 16:05
Native speaker of: Native in SwedishSwedish
PRO pts in pair: 1498
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55 mins

55 mins peer agreement (net): +1
peroxydase induced color change


Explanation:
vermitteln = to indicate, to put in evidence.
Umsetzung = displacement, transformation
Farbstoff = colorant, pigment

Farbstoffumsetzung, can be interpreted as a colorant migration or a colorant transformation, that will change it's color. I prefer the second hypothesis because peroxydation generally have discoloring effects on organic material. From it's name, a peroxydase is an enzym that will induce such an oxydation, thus induce such a color change.
I think that "vermittelten" is not used exactly in the sense of indicating, but rather in the sense of revealing.
A literal translation would be
"peroxydase indicated colorchange


    25 years in analytical chemistry instrumentation
Yves Georges
France
Local time: 16:05
Native speaker of: Native in FrenchFrench
PRO pts in pair: 185
Grading comment
Thank you, I think I like your answer best!

Peer comments on this answer (and responses from the answerer)
agree  mckinnc: I just think that's it
15 mins
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1 hr
peroxidase-mediated color change


Explanation:
peroxidase-mediated color (or chromatic) change

Reuter-Reuter Medizinisches Wörterbuch ISBN 3 13 100491 6

HTH Tom


    see above
Tom Funke
Local time: 10:05
Native speaker of: English
PRO pts in pair: 2419
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1 hr
peroxidase-mediated color conversion


Explanation:
In a biochemical context like yours, vermittelt = mediated. Also, I am not entirely convinced that Farbstoffumsetzung = color change, Umsetzung is generally conversion or transformation [Reuter & Reuter, Medizin, and Kucera, Chemie]

Kucera: Dictionary of Chemistry English-German : peroxidase n (Biochem) / Peroxidase f (eine Oxidoreduktase)
Kucera: Dictionary of Chemistry German-English : Peroxidase f (eine Oxidoreduktase) (Biochem) / peroxidase n

The following reference did originate in Germany, but its English is quite impeccable.
...were labelled via specific antibodies and enzyme-mediated color conversion.
www.biometra.de/pages/inhalt/inhalt_applications_i_tankblot...

Any change in the bar code applied to ... use of the recently introduced peroxydase-mediated deposition of hapten- or ...
www.wiley.co.uk/products/subject/life/cytometry/ISAC96/ac7....

... quantitative RT-PCR coupled with an enzyme-linked immunosorbent assay as described ... sets of color codes. A color conversion panel shown at the bottom ...
www.dna-res.kazusa.or.jp/8/2/05/HTMLS/node3.html

Some further references that may or may not be of help:
www.cbs.umn.edu/CGC/Bibliography/4401.txt
pondside.uchicago.edu/ecol-evol/faculty/ruddat_m.html
www.uth.tmc.edu/apstracts/1998/lung/March/47L.html
www.phm.auckland.ac.nz/student_resources/530203/lectures/ 530203_cumulative_drug_responses.nh.htm

HTH!



    Reuter & Reuter, Medical Dictionary
    Kucera, WB Chemie
Ulrike Lieder
Local time: 07:05
Native speaker of: German
PRO pts in pair: 3525
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