LAL
English translation: limulus amebocyte lysate
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| 00:27 Aug 30, 2014 |
| German to English translations [PRO] Medical - Medical: Pharmaceuticals | |||||||
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|  Selected response from: Donald Jacobson United States Local time: 07:09 | ||||||
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| 5 +1 | limulus amebocyte lysate |
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| Discussion entries: 6 | |
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39 mins confidence: 
peer agreement (net): +1
peer agreement (net): +1limulus amebocyte lysate Explanation: Limulus-Amoebozytenlysat: reference:Der Grosse Reuter Springer Universalwoerteruch Medizin, Pharmakologie, Dentistry Limulus amebocyte lysate, an aequous extract of amebocytes from the horseshoe crab (Limulus polyphemus), is commonly used in a test to detect bacterial endotoxins. Wikipedia: -------------------------------------------------- Note added at 13 hrs (2014-08-30 13:33:02 GMT) -------------------------------------------------- http://www.piercenet.com/product/pierce-lal-chromogenic-endo... Endotoxin Quantitation Reaction Scheme Pierce LAL Chromogenic Endotoxin Quantitation Kit reaction scheme. A small volume of the sample (10μL) is combined with the Limulus Amebocyte Lysate, and endotoxins in the sample activate the proteolytic activity of Factor C. When the chromogenic substrate is added, the activated protease catalyzes the cleavage of p-nitroalinine (pNA), resulting in yellow color that can be quantitated by measuring the absorbance at 405nm (A405) and extrapolating against a standard curve. You may also need 39000 - Lane Marker Reducing Sample Buffer 78420 - Halt Phosphatase Inhibitor Cocktail 21327 - EZ-Link Sulfo-NHS-LC-Biotin, No-Weigh Format 89870 - Pierce C18 Spin Columns 21009 - IgG Elution Buffer |
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